Construct: ORF TRCN0000467773
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000251.1_s317c1
- Derived from:
- ccsbBroadEn_00646
- DNA Barcode:
- ACGCCGAGATCCGCATAAGCCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GLO1 (2739)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467773
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2739 | GLO1 | glyoxalase I | NM_006708.3 | 100% | 100% | |
| 2 | mouse | 109801 | Glo1 | glyoxalase 1 | NM_001113560.1 | 82.4% | 90.2% | (many diffs) |
| 3 | mouse | 109801 | Glo1 | glyoxalase 1 | NM_025374.3 | 82.4% | 90.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 618
- ORF length:
- 552
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agaaccgcag cccccgtccg gcggcctcac ggacgaggcc gccctcagtt 121 gctgctccga cgcggacccc agtaccaagg attttctatt gcagcagacc atgctacgag 181 tgaaggatcc taagaagtca ctggattttt atactagagt tcttggaatg acgctaatcc 241 aaaaatgtga ttttcccatt atgaagtttt cactctactt cttggcttat gaggataaaa 301 atgacatccc taaagaaaaa gatgaaaaaa tagcctgggc gctctccaga aaagctacac 361 ttgagctgac acacaattgg ggcactgaag atgatgagac ccagagttac cacaatggca 421 attcagaccc tcgaggattc ggtcatattg gaattgctgt tcctgatgta tacagtgctt 481 gtaaaaggtt tGAAGAACTG GGAGTCAAAT TTGTGAAGAA ACCTGATGAT GGTAAAATGA 541 AAGGCCTGGC ATTTATTCAA GATCCTGATG GCTACTGGAT TGAAATTTTG AATCCTAACA 601 AAATGGCAAC CTTAATGTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 661 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 721 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACGCCGA GATCCGCATA 781 AGCCGTAACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt