Construct: ORF TRCN0000467817
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017662.1_s317c1
- Derived from:
- ccsbBroadEn_01772
- DNA Barcode:
- CGAAGACCTACAAATAAACATGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- VIP (7432)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467817
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7432 | VIP | vasoactive intestinal peptide | NM_194435.3 | 100% | 100% | |
| 2 | human | 7432 | VIP | vasoactive intestinal peptide | NM_003381.4 | 99.4% | 99.4% | 334_336delAGC |
| 3 | human | 7432 | VIP | vasoactive intestinal peptide | XM_005267135.3 | 99.4% | 99.4% | 462_463insAGC |
| 4 | human | 7432 | VIP | vasoactive intestinal peptide | XM_006715562.4 | 98.8% | 98.8% | 334_336delAGC;465_466insAGC |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 573
- ORF length:
- 507
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga caccagaaat aaggcccagc tccttgtgct cctgactctt ctcagtgtgc 121 tcttctcaca gacttcggca tggcctcttt acagggcacc ttctgctctc aggttgggtg 181 acagaatacc ctttgaggga gcaaatgaac cTGATCAAGT TTCATTAAAA GAAGACATTG 241 ACATGTTGCA AAATGCATTA GCTGAAAATG ACACACCCTA TTATGATGTA TCCAGAAATG 301 CCAGGCATGC TGATGGAGTT TTCACCAGTG ACTTCAGTAA ACTCTTGGGT CAACTTTCTG 361 CCAAAAAGTA CCTTGAGTCT CTTATGGGAA AACGTGTTAG TAACATCTCA GAAGACCCTG 421 TACCAGTCAA ACGTCACTCA GATGCAGTCT TCACTGACAA CTATACCCGC CTTAGAAAAC 481 AAATGGCTGT AAAGAAATAT TTGAACTCAA TTCTGAATGG AAAGAGGAGC AGTGAGGGAG 541 AATCTCCCGA CTTTCCAGAA GAGTTAGAAA AATGCCCAAC TTTCTTGTAC AAAGTGGTTG 601 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 661 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACG 721 AAGACCTACA AATAAACATG TCACGCGTTA AGTCgacaat caacctctgg attacaaaat 781 ttgtgaaaga tt