Construct: ORF TRCN0000467819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012549.3_s317c1
- Derived from:
- ccsbBroadEn_08054
- DNA Barcode:
- TACCCCACCAGTGGGTCCTTGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EIF2AK1 (27102)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 27102 | EIF2AK1 | eukaryotic translation init... | NM_014413.4 | 99.8% | 99.8% | 918G>A;1877A>C |
| 2 | human | 27102 | EIF2AK1 | eukaryotic translation init... | NM_001134335.1 | 99.7% | 99.6% | 728_729insAGC;915G>A;1874A>C |
| 3 | human | 27102 | EIF2AK1 | eukaryotic translation init... | XM_024446718.1 | 66.8% | 66.8% | 0_1ins624;294G>A;1253A>C |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1956
- ORF length:
- 1890
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca ggggggcaac tccggggtcc gcaagcgcga agaggagggc gacggggctg 121 gggctgtggc tgcgccgccg gccatcgact ttcccgccga gggcccggac cccgaatatg 181 acgaatctga tgttccagca gaaatccagg tgttaaaaga acccctacaa cagccaacct 241 tcccttttgc agttgcaaac caactcttgc tggtttcttt gctggagcac ttgagccacg 301 tgcatgaacc aaacccactt cgttcaagac aggtgtttaa gctactttgc cagacgttta 361 tcaaaatggg gctgctgtct tctttcactt gtagtgacga gtttagctca ttgagactac 421 atcacaacag agctattact cacttaatga ggtctgctaa agagagagtt cgtcaggatc 481 cttgtgagga tatttctcgt atccagaaaa tcagatcaag ggaagtagcc ttggaagcac 541 aaacttcacg ttacttaaat gaatttgaag aacttgccat cttaggaaaa ggtggatacg 601 gaagagtata caaggtcagg aataaattag atggtcagta ttatgcaata aaaaaaatcc 661 tgattaaggg tgcaactaaa acagtttgca tgaaggtcct acgggaagtg aaggtgctgg 721 caggtcttca gcaccccaat attgttggct atcacaccgc gtggatagaa catgttcatg 781 tgattcagcc acgagcagac agagctgcca ttgagttgcc atctctggaa gtgctctccg 841 accaggaaga ggacagagag caatgtggtg ttaaaaatga tgaaagtagc agctcatcca 901 ttatctttgc tgagcccacc ccagaaaaag aaaaacgctt tggagaatct gacactgaaa 961 atcagaataa caagtcggtg aaatacacca ccaatttagt cataagagaa tctggtgaac 1021 ttgagtcgac cctggagctc caggaaaatg gcttggctgg tttgtctgcc agttcaattg 1081 tggaacagca gctgccactc aggcgtaatt cccacctaga ggagagtttc acatccaccg 1141 aagaatcttc cgaagaaaat gtcaactttt tgggtcagac agaggcacag taccacctga 1201 tgctgcacat ccagatgcag ctgtgtgagc tctcgctgtg ggattggata gtcgagagaa 1261 acaagcgggg ccgggagtat gtggacgagt ctgcctgtcc ttatgttatg gccaatgttg 1321 caacaaaaat ttttcaagaa ttggtagaag gtgtgtttta catacataac atgggaattg 1381 tgcaccgaga tctgaagcca agaaatattt ttcttcatgg ccctgatcag caagtaaaaa 1441 taggagactt tggtctggcc tgcacagaca tcctacagaa gaacacagac tggaccaaca 1501 gaaacgggaa gagaacacca acacatacgt ccagagtggg tacttgtctg tacgcttcac 1561 ccgaacagtt ggaaggatct gagtatgatg ccaagtcaga tatgtacagc ttgggtgtgg 1621 tcctgctaga gctctttcag ccgtttggaa cagaaatgga gcgagcagaa gttctaacag 1681 gtttaagaac tggtcagttg ccggaaTCCC TCCGTAAAAG GTGTCCAGTG CAAGCCAAGT 1741 ATATCCAGCA CTTAACGAGA AGGAACTCAT CGCAGAGACC ATCTGCCATT CAGCTGCTGC 1801 AGAGTGAACT TTTCCAAAAT TCTGGAAATG TTAACCTCAC CCTACAGATG AAGATAATAG 1861 AGCAAGAAAA AGAAATTGCA GAACTAAAGA AGCAGCTAAA CCTCCTTTCT CAAGACAAAG 1921 GGGTGAGGGA TGACGGAAAG GCTGGGGGCG TGGGATGCCC AACTTTCTTG TACAAAGTGG 1981 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2041 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2101 ATACCCCACC AGTGGGTCCT TGTAAACGCG TTAAGTCgac aatcaacctc tggattacaa 2161 aatttgtgaa agatt