Construct: ORF TRCN0000467843
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010663.1_s317c1
- Derived from:
- BRDN0000395157
- DNA Barcode:
- CTAGGCATTTACTTGCGCACACGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHX57 (90957)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467843
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 90957 | DHX57 | DExH-box helicase 57 | NM_198963.3 | 99.7% | 99.7% | (many diffs) |
| 2 | human | 90957 | DHX57 | DExH-box helicase 57 | XM_011533154.2 | 99.7% | 99.7% | (many diffs) |
| 3 | human | 90957 | DHX57 | DExH-box helicase 57 | XM_011533155.2 | 99.7% | 99.7% | (many diffs) |
| 4 | human | 90957 | DHX57 | DExH-box helicase 57 | XM_011533156.3 | 96.5% | 96.3% | (many diffs) |
| 5 | human | 90957 | DHX57 | DExH-box helicase 57 | NM_001329963.1 | 92.4% | 92.4% | (many diffs) |
| 6 | human | 90957 | DHX57 | DExH-box helicase 57 | XM_024453215.1 | 92.4% | 92.4% | (many diffs) |
| 7 | human | 90957 | DHX57 | DExH-box helicase 57 | XR_002959363.1 | 90.1% | (many diffs) | |
| 8 | human | 90957 | DHX57 | DExH-box helicase 57 | XR_001739030.2 | 83.8% | (many diffs) | |
| 9 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | NM_001163759.1 | 83.9% | 86.6% | (many diffs) |
| 10 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | XM_006523447.1 | 83.9% | 86.6% | (many diffs) |
| 11 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | NM_198942.2 | 80.9% | 83.2% | (many diffs) |
| 12 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | XM_006523448.1 | 80.9% | 83.2% | (many diffs) |
| 13 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | XM_006523449.3 | 79.8% | 82.2% | (many diffs) |
| 14 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | XM_006523451.1 | 70.8% | 73.7% | (many diffs) |
| 15 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | XM_017317203.1 | 53.3% | 57.7% | (many diffs) |
| 16 | mouse | 106794 | Dhx57 | DEAH (Asp-Glu-Ala-Asp/His) ... | XM_011246245.2 | 39.5% | 43.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4227
- ORF length:
- 4158
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagttcttca gtaagaagaa aaggcaagcc aggcaaagga ggtggaaaag 121 ggtcttctag aggaggaaga ggaggcagga gtcacgccag taaatctcat gggagtggtg 181 gcggtggcgg tggtggtggt ggtggaggtg gcggcaacag aaaggcctcc agtagaatat 241 gggatgatgg agatgacttt tgtatcttca gtgaatcaag gcgcccttcc agacctagca 301 acagtaacat aagcaaagga gagtcacgcc caaaatggaa acccattccc aaagtacccc 361 ttcagactct acatatgact tctgagaatc aagagaaagt gaaagctctt ctccgagacc 421 tgcaagaaca agatgctgat gctggatctg aaagaggcct ttctggggag gaggaagatg 481 atgagcctga ttgctgtaac gatgagcggt actggccagc tggacaggaa ccttccctcg 541 ttcccgactt ggatcctttg gaatatgctg gcttagcctc agtggagcct tatgttccag 601 aatttacagt ctccccattt gcagtgcaaa aactttccag gtatggtttc aatactgaac 661 gctgtcaagc ggtcctgagg atgtgtgatg gagatgtggg agcatcacta gagcatctcc 721 ttacccagtg tttttcagag acatttggag agaggatgaa gatctctgag gcagtcaacc 781 agataagctt ggatgagtgt atggaacagc gacaggaaga ggcatttgct ctcaagtcca 841 tctgtggaga aaaatttata gaaagaattc agaacagagt ctggaccatt gggttagaac 901 tggagtatct gacaagtaga ttccgcaaat ccaagccaaa agaaagtacc aaaaatgtac 961 aagagaattc acttgaaatc tgtaaatttt acctcaaagg aaattgtaaa tttggatcaa 1021 aatgcagatt caaacatgaa gtgcccccaa atcaaattgt tggaagaata gaaagaagtg 1081 tagatgattc tcatcttaat gctattgaag atgcatcttt tttatatgaa cttgaaattc 1141 gattttctaa agaccacaaa tatccctacc aagctccgct cgtggcattt tattccacca 1201 atgagaacct acctctggct tgtcgtttac atatttctga gtttctttat gacaaggcct 1261 tgacatttgc ggaaacttcg gaacctgtcg tatattcttt gataaccctt ttagaggaag 1321 agtcggaaat agtcaagtta ctaacgaata cccaccacaa gtatagtgac cctcctgtga 1381 actttctgcc agtaccctct aggaccagaa taaataatcc tgcctgtcat aaaacagtga 1441 ttccaaataa ttcttttgtt tctaatcaaa ttccagaagt tgaaaaagca tcagaatctg 1501 aggagtcaga tgaggatgac ggtcctgcac ctgttatagt agagaatgaa agctatgtga 1561 accttaagaa aaagatttcc aaaagatatg actggcaggc aaagtcagta catgctgaaa 1621 atggtaaaat ctgcaagcag ttccgaatga aacaggcttc cagacagttc cagtccattc 1681 tgcaagagag gcaatcactc cctgcttggg aagaaagaga aaccattctt aacttattgc 1741 gtaagcacca ggtggttgtc ataagtggta tgactggatg tgggaaaacc acacaaattc 1801 cgcagtttat tctggatgat tctctgagtg gaccacctga gaaggtagcc aacatcatct 1861 gtacccaacc ccgacgaatc tctgcaatct ctgttgctga acgcgttgct aaagaaagag 1921 cagagagggt gggtctgacc gtgggatacc agattcggtt agaaagtgtc aagtcctcag 1981 ccaccagact gttatactgc accacgggag tgctgctgag aaggctagaa ggagatacag 2041 ctctacaagg agtttcccat atcattgttg atgaagttca tgagaggaca gaagaaagtg 2101 acttcttgct gctagttttg aaggacattg tatcgcagag gccaggtctt caagttattt 2161 taatgagtgc aactctaaac gctgagcttt tttcagacta ttttaattcc tgccccgtta 2221 ttactatacc aggtcgtaca tttcctgttg atcaattttt tttggaagat gcaattgctg 2281 tgacaaggta tgtattacag gatgggagcc catatatgcg gtccatgaaa cagatttcaa 2341 aggaaaagct taaagcaagg cggaacagaa ctgcatttga agaagtggaa gaagacctaa 2401 ggctctccct tcacctccag gatcaggatt ctgtcaaaga tgcagtgcca gatcaacagt 2461 tagattttaa gcagctcctg gcccgctata aaggggttag caagtcagtc atcaaaacaa 2521 tgtccatcat ggattttgaa aaggtgaatc ttgaattaat agaggcctta ttagagtgga 2581 ttgtggatgg aaagcactcc taccctccag gtgctatact tgtattttta ccaggactag 2641 cagaaatcaa aatgctttat gaacagctac agtctaattc tcttttcaac aacagacgta 2701 gtaatcgatg tgttattcac ccacttcatt catctttatc cagtgaagag cagcaggctg 2761 tgtttgtaaa acctcctgca ggagtaacta agattataat ttccaccaac attgctgaga 2821 catccataac catcgatgat gttgtctatg ttatcgattc tgggaaaatg aaagaaaaga 2881 gatatgatgc cagcaaaggg atggaaagtc tagaggacac ctttgtatct caagctaatg 2941 ctctacaaag gaaaggccga gcaggccgtg ttgcatctgg ggtctgcttc catttattca 3001 ctagccatca ctacaatcac cagcttttaa aacaacagct accagaaata caaagagtgc 3061 cattggaaca gctgtgtcta agaattaaaa ttttagagat gtttagtgct cataatctcc 3121 agtctgtgtt ctctcggctc attgaacctc cacacaccga ttctcttcgt gcctcaaaaa 3181 tacgattacg agacttagga gcattaactc cagatgaaag attgacccct cttgggtatc 3241 atttggcctc tctgcccgtg gatgtgagaa ttggcaaact aatgttgttt gggtctccat 3301 tccgctgttt ggatcctgct ctcaccattg ctgccagttt ggcttttaag tcgccgtttg 3361 tatctccctg ggataaaaaa gaagaagcta accagaaaaa gctggaattt gcattcgcaa 3421 acagtgatta tctggccctt ctacaagcgt ataagggatg gcagctaagt acaaaagaag 3481 gcgtgcgtgc aagttataat tactgcagac aaaacttctt gtctggaaga gttctgcagg 3541 aaatggccag cctcaaacga caattcacgg aactgttatc ggatataggg tttgcaaggg 3601 aagggctcag agcaagggaa attgagaaaa gggcccaagg aggagatggt gtcttagatg 3661 ccacaggaga agaggcaaac tcaaatgctg agaaccccaa gctgatatca gcaatgctgt 3721 gtgctgcttt gtatccaaat gtagtgcagg tgaaaagccc agaaggaaaa tttcagaaga 3781 ccagtactgg agctgtcaga atgcaaccaa aatcagctga gttgaagttt gtcaccaaga 3841 acgatGGATA TGTACACATT CACCCTTCAT CAGTGAACTA TCAGGTGAGA CACTTTGACA 3901 GCCCCTACCT GTTGTACCAC GAGAAGATAA AAACTAGTCG AGTATTCATC CGAGACTGCA 3961 GCATGGTGTC TGTGTACCCG CTGGTCTTGT TTGGAGGAGG CCAAGTGAAT GTGCAGCTTC 4021 AAAGAGGAGA GTTCGTTGTC TCCCTGGATG ATGGTTGGAT CCGTTTTGTA GCTGCTTCCC 4081 ATCAGGTGGC TGAACTGGTA AAGGAGCTTC GTTGTGAACT TGATCAGCTT CTCCAGGATA 4141 AAATTAAAAA CCCAAGCATT GATCTGTGTA CGTGTCCTCG AGGATCCCGG ATCATCAGCA 4201 CAATTGTGAA ACTTGTCACC ACACAATTGC CAACTTTCTT GTACAAAGTG GTTGATATCG 4261 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 4321 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACTAGGCAT 4381 TTACTTGCGC ACACGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 4441 aagatt