Construct: ORF TRCN0000467875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002912.1_s317c1
- Derived from:
- ccsbBroadEn_02722
- DNA Barcode:
- GCGATCTCTACATATGCGCAAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- COLGALT2 (23127)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467875
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23127 | COLGALT2 | collagen beta(1-O)galactosy... | NM_015101.4 | 100% | 100% | |
| 2 | human | 23127 | COLGALT2 | collagen beta(1-O)galactosy... | NM_001303420.2 | 87.5% | 85.1% | (many diffs) |
| 3 | human | 23127 | COLGALT2 | collagen beta(1-O)galactosy... | NM_001303421.2 | 80.8% | 80.8% | 0_1ins360 |
| 4 | mouse | 269132 | Colgalt2 | collagen beta(1-O)galactosy... | NM_177756.4 | 88.5% | 92.3% | (many diffs) |
| 5 | mouse | 269132 | Colgalt2 | collagen beta(1-O)galactosy... | XR_001784706.1 | 37% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1944
- ORF length:
- 1878
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcgcgccct gctgccaccc tcgcctggtc gctactgctc ctctcctcag 121 ccctgctccg cgaaggctgc cgagcgcgct tcgtcgccga gcgggactcg gaggacgacg 181 gagaggagcc ggtggttttc ccggagtcgc ccctgcagag ccccacggtg ctcgtggcgg 241 tcctcgcccg caacgcggcg cacacgctgc cgcacttcct cggctgcctg gagcggctgg 301 actaccccaa gagcaggatg gccatctggg cagccactga tcacaatgtg gataatacaa 361 cagaaatatt cagggagtgg ttgaaaaatg tacagagact ctatcactat gtggagtgga 421 ggcctatgga tgaaccagag tcttaccctg atgaaattgg accaaagcac tggccaacct 481 cccggtttgc ccatgtgatg aaactacgac aggcagccct tcgaactgcg agggaaaaat 541 ggtcagacta cattctgttc atagatgttg acaatttcct gactaatcca cagaccctca 601 atctactgat tgcagaaaac aaaactattg tggcccccat gctggagtct cggggcctgt 661 attctaattt ctggtgcgga atcaccccta agggcttcta taagaggacc ccagactacg 721 ttcagattcg agaatggaag aggacaggct gcttccccgt ccccatggtc cactccacct 781 tcctaattga cctcaggaag gaggcctcgg acaagctgac tttctacccc ccacaccagg 841 actacacctg gacctttgat gacatcattg tctttgcctt ctccagcagg caagcaggca 901 tccagatgta cctctgcaac agagagcact atggctacct gcccatcccc ctgaagcccc 961 atcagacact gcaggaagac atcgagaacc tcatccatgt gcagattgaa gcaatgattg 1021 accgtcctcc aatggaaccc tcccagtatg tctcagttgt ccctaaatat ccagacaaga 1081 tgggatttga tgagattttc atgataaacc tcaaacgcag aaaggacagg cgggaccgga 1141 tgctgcgcac actgtatgaa caggagattg aggtcaagat tgtcgaggct gtggatggaa 1201 aggcactcaa cacaagccag ctgaaggcac tgaatattga aatgctgcct ggctatcgag 1261 atccctattc ctccaggcct ctaacaaggg gtgaaatcgg ctgctttctc agccactact 1321 cagtctggaa agaggtaatt gatcgagagc tagagaagac tcttgtaatt gaagacgatg 1381 tgcgttttga gcatcagttt aagaagaagc tgatgaagct gatggataac attgaccagg 1441 ctcagctgga ctgggaactg atttatattg gtaggaagag gatgcaagta aaggagccag 1501 agaaagcagt gcccaatgtg gcaaacctgg tcgaagccga ctattcctac tggaccctgg 1561 gctacgtcat ctctctggaa ggagcacaga agctggttgg agccaaTCCT TTTGGGAAGA 1621 TGCTGCCAGT GGATGAGTTT CTGCCAGTCA TGTACAACAA GCATCCCGTA GCCGAGTACA 1681 AGGAGTATTA TGAATCCAGG GACCTGAAAG CCTTCTCTGC AGAACCCTTG CTCATCTACC 1741 CTACGCACTA CACAGGCCAG CCGGGGTACC TGAGTGACAC GGAGACCTCC ACCATCTGGG 1801 ACAATGAGAC AGTGGCCACC GACTGGGATA GGACACATGC CTGGAAGTCC CGGAAGCAAA 1861 GCCGCATCTA CAGCAATGCC AAGAACACAG AGGCCCTGCC ACCGCCAACC TCCCTGGACA 1921 CTGTGCCTTC AAGGGATGAG CTATGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1981 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 2041 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG CGATCTCTAC 2101 ATATGCGCAA GGTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 2161 att