Transcript: Mouse XR_001784706.1

PREDICTED: Mus musculus collagen beta(1-O)galactosyltransferase 2 (Colgalt2), transcript variant X1, misc_RNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Colgalt2 (269132)
Length:
4503
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001784706.1
NBCI Gene record:
Colgalt2 (269132)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XR_001784706.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000217789 CTAACTTCTGGTGCGGAATTA pLKO.1 929 3UTR 100% 13.200 18.480 N Colgalt2 n/a
2 TRCN0000216907 GTGGACAATACAACGGAAATA pLKO.1 613 3UTR 100% 13.200 18.480 N Colgalt2 n/a
3 TRCN0000428579 ACTAATCTAAGTGGTCCATTT pLKO_005 2977 3UTR 100% 10.800 15.120 N Colgalt2 n/a
4 TRCN0000182766 CCAGAGTCTTACCCTGATGAA pLKO.1 700 3UTR 100% 4.950 6.930 N Colgalt2 n/a
5 TRCN0000182245 CCCTGATGAAATCGGACCAAA pLKO.1 711 3UTR 100% 4.950 6.930 N Colgalt2 n/a
6 TRCN0000182429 GCGCATCTGAATTGCCTGAAT pLKO.1 3728 3UTR 100% 4.950 6.930 N Colgalt2 n/a
7 TRCN0000198900 GTAAATCTCGTCGAAGCTGAT pLKO.1 2171 3UTR 100% 4.050 5.670 N Colgalt2 n/a
8 TRCN0000181907 GCAGTGCCCAATGTTGTAAAT pLKO.1 2156 3UTR 100% 13.200 10.560 N Colgalt2 n/a
9 TRCN0000216853 GTCGACAACTTCCTGACTAAT pLKO.1 832 3UTR 100% 13.200 10.560 N Colgalt2 n/a
10 TRCN0000424517 TGCAGCGTTTGTATCACTATG pLKO_005 656 3UTR 100% 10.800 8.640 N Colgalt2 n/a
11 TRCN0000178342 GCTGAGTACAAGGAGTATTAT pLKO.1 2321 3UTR 100% 15.000 10.500 N Colgalt2 n/a
12 TRCN0000181826 GCCTGGCTTTAACTGTGTATT pLKO.1 4237 3UTR 100% 13.200 9.240 N Colgalt2 n/a
13 TRCN0000182206 CCTGTGCAACAAGGAACACTA pLKO.1 1176 3UTR 100% 4.950 3.465 N Colgalt2 n/a
14 TRCN0000034743 GCCACTGATCACAATGTGGAT pLKO.1 598 3UTR 100% 2.640 1.848 N COLGALT2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001784706.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02722 pDONR223 100% 37% None (many diffs) n/a
2 ccsbBroad304_02722 pLX_304 0% 37% V5 (many diffs) n/a
3 TRCN0000467875 GCGATCTCTACATATGCGCAAGGT pLX_317 18.3% 37% V5 (many diffs) n/a
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