Construct: ORF TRCN0000467893
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004522.1_s317c1
- Derived from:
- ccsbBroadEn_01200
- DNA Barcode:
- TCAGGCGGTGCCCGAAGAAGGGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PIGH (5283)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467893
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5283 | PIGH | phosphatidylinositol glycan... | NM_004569.5 | 100% | 100% | |
| 2 | human | 5283 | PIGH | phosphatidylinositol glycan... | NM_001363694.1 | 99.4% | 99.4% | 389_390insGCA |
| 3 | human | 5283 | PIGH | phosphatidylinositol glycan... | XM_011536838.3 | 84.9% | 80.6% | (many diffs) |
| 4 | human | 5283 | PIGH | phosphatidylinositol glycan... | XM_017021371.2 | 84.4% | 80.1% | (many diffs) |
| 5 | mouse | 110417 | Pigh | phosphatidylinositol glycan... | NM_029988.2 | 87.2% | 88.2% | (many diffs) |
| 6 | mouse | 110417 | Pigh | phosphatidylinositol glycan... | XM_011243987.2 | 76.9% | 77.8% | (many diffs) |
| 7 | mouse | 110417 | Pigh | phosphatidylinositol glycan... | XM_017314937.1 | 34.4% | 24.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 630
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga ggatgagcgg agcttttcgg atatctgcgg cggccgcctg gcgctgcagc 121 gccgctacta ctccccgtcc tgccgggaat tctgcctcag ctgccctcgg ctctcgctgc 181 gttcgctcac cgctgtcacc tgcacggtgt ggctggcggc ctacggactc ttcacccTCT 241 GCGAGAACAG CATGATCCTC TCTGCTGCCA TCTTCATCAC CCTCTTAGGT CTGCTTGGTT 301 ATCTCCATTT TGTGAAGATT GATCAGGAGA CTCTGTTAAT CATTGATTCC CTTGGCATTC 361 AGATGACTTC ATCTTATGCT TCAGGCAAAG AAAGCACTAC CTTCATAGAA ATGGGCAAGG 421 TCAAGGATAT TGTCATCAAT GAGGCCATTT ACATGCAGAA GGTGATTTAC TACCTCTGCA 481 TCTTATTGAA AGATCCAGTG GAACCACATG GGATATCCCA AGTAGTACCC GTCTTCCAGA 541 GTGCCAAGCC CCGGCTGGAC TGCTTGATTG AAGTATACAG GAGCTGCCAG GAGATCCTGG 601 CACACCAGAA AGCCACATCA ACAAGCCCAT GCCCAACTTT CTTGTACAAA GTGGTTGATA 661 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 721 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGATCAGG 781 CGGTGCCCGA AGAAGGGCCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 841 tgaaagatt