Construct: ORF TRCN0000467905

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006789.1_s317c1
Derived from:
ccsbBroadEn_03291
DNA Barcode:
TCAATGGACAGATCTATTCAAGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
POMP (51371)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000467905
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51371 POMP proteasome maturation protein NM_015932.6 100% 100%
2 mouse 66537 Pomp proteasome maturation protein NM_025624.2 86% 95% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
489
ORF length:
423
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa tgccagagga cttggatctg agctaaagga cagtattcca gttactgaac
121ttTCAGCAAG TGGACCTTTT GAAAGTCATG ATCTTCTTCG GAAAGGTTTT TCTTGTGTGA
181AAAATGAACT TTTGCCTAGT CATCCCCTTG AATTATCAGA AAAAAATTTC CAGCTCAACC
241AAGATAAAAT GAATTTTTCC ACACTGAGAA ACATTCAGGG TCTATTTGCT CCGCTAAAAT
301TACAGATGGA ATTCAAGGCA GTGCAGCAGG TTCAGCGTCT TCCATTTCTT TCAAGCTCAA
361ATCTTTCACT GGATGTTTTG AGGGGTAATG ATGAGACTAT TGGATTTGAG GATATTCTTA
421ATGATCCATC ACAAAGCGAA GTCATGGGAG AGCCACACTT GATGGTGGAA TATAAACTTG
481GTTTACTGTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC
541CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA
601TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATCAATG GACAGATCTA TTCAAGGCAC
661GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt

Download FASTA (ORF) (Full)