Construct: ORF TRCN0000467921
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005895.1_s317c1
- Derived from:
- ccsbBroadEn_00415
- DNA Barcode:
- TACCAGCGCCTATCAGGGCTACAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYP24A1 (1591)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467921
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1591 | CYP24A1 | cytochrome P450 family 24 s... | NM_001128915.2 | 100% | 100% | |
| 2 | human | 1591 | CYP24A1 | cytochrome P450 family 24 s... | XM_017027693.2 | 100% | 100% | |
| 3 | human | 1591 | CYP24A1 | cytochrome P450 family 24 s... | NM_000782.5 | 87.1% | 87.1% | 1237_1434del |
| 4 | human | 1591 | CYP24A1 | cytochrome P450 family 24 s... | XM_005260304.5 | 87.1% | 87.1% | 1237_1434del |
| 5 | human | 1591 | CYP24A1 | cytochrome P450 family 24 s... | XM_017027691.2 | 87.1% | 87.1% | 1237_1434del |
| 6 | human | 1591 | CYP24A1 | cytochrome P450 family 24 s... | XM_017027692.2 | 87.1% | 87.1% | 1237_1434del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1413
- ORF length:
- 1344
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagctccccc atcagcaaga gccgctcgct tgccgccttc ctgcagcagc 121 tgcgcagtcc gaggcagccc ccgagactgg tgacatctac ggcgtacacg tcccctcagc 181 cgcgagaggt gccagtctgc ccgctgacag ctggtggcga gactcagaac gcggccgccc 241 tgccgggccc caccagctgg ccactgctgg gcagcctgct gcagattctc tggaaagggg 301 gtctcaagaa acagcacgac accctggtgg agtaccacaa gaagtatggc aagattttcc 361 gcatgaagtt gggttccttt gagtcggtgc acctgggctc gccatgcctg ctggaagcgc 421 tgtaccgcac cgagagcgcg tacccgcagc ggctggagat caaaccgtgg aaggcctatc 481 gcgactaccg caaagaaggc tacgggctgc tgatcctgga aggggaagac tggcagcggg 541 tccggagtgc ctttcaaaag aaactaatga aaccagggga agtgatgaag ctggacaaca 601 aaatcaatga ggtcttggcc gattttatgg gcagaataga tgagctctgt gatgaaagag 661 gccacgttga agacttgtac agcgaactga acaaatggtc gtttgaaagt atctgcctcg 721 tgttgtatga gaagagattt gggcttctcc agaagaatgc aggggatgaa gctgtgaact 781 tcatcatggc catcaaaaca atgatgagca cgtttgggag gatgatggtc actccagtcg 841 agctgcacaa gagcctcaac accaaggtct ggcaggacca cactctggcc tgggacacca 901 ttttcaaatc agtcaaagct tgtatcgaca accggttaga gaagtattct cagcagccta 961 gtgcagattt cctttgtgac atttatcacc agaatcggct ttCAAAGAAA GAATTGTATG 1021 CTGCTGTCAC AGAGCTCCAG CTGGCTGCGG TGGAAACGAC AGCAAACAGT CTAATGTGGA 1081 TTCTCTACAA TTTATCCCGT AATCCCCAAG TGCAACAAAA GCTTCTTAAG GAAATTCAAA 1141 GTGTATTACC TGAGAATCAG GTGCCACGGG CAGAAGATTT GAGGAATATG CCGTATTTAA 1201 AAGCCTGTCT GAAAGAATCT ATGAGGCTTA CGCCGAGTGT ACCATTTACA ACTCGGACTC 1261 TTGACAAGGC AACAGTTCTG GGTGAATATG CTTTACCCAA AGGAATTGTC CGCAAATACG 1321 ACATCCAGGC CACAGACAAT GAGCCTGTTG AGATGCTACA CTCAGGCACC CTGGTGCCCA 1381 GCCGGGAACT CCCCATCGCG TTTTGCCAGC GATTGCCAAC TTTCTTGTAC AAAGTGGTTG 1441 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 1501 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGATA 1561 CCAGCGCCTA TCAGGGCTAC ATACGCGTTA AGTCgacaat caacctctgg attacaaaat 1621 ttgtgaaaga tt