Construct: ORF TRCN0000467965
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004745.1_s317c1
- Derived from:
- ccsbBroadEn_00336
- DNA Barcode:
- CAGTAAACTGGAACATCCGATTTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNGA3 (1261)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467965
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1261 | CNGA3 | cyclic nucleotide gated cha... | NM_001298.3 | 100% | 100% | |
| 2 | human | 1261 | CNGA3 | cyclic nucleotide gated cha... | NM_001079878.2 | 97.4% | 97.2% | 395_396ins54 |
| 3 | human | 1261 | CNGA3 | cyclic nucleotide gated cha... | XM_011510554.2 | 92.6% | 92.6% | 213_377del |
| 4 | human | 1261 | CNGA3 | cyclic nucleotide gated cha... | XM_006712243.2 | 90.2% | 90.1% | 213_377del;560_561ins54 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2151
- ORF length:
- 2082
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaagatc aacacccaat actcccaccc ctccaggacc cacctcaagg 121 taaagacctc agaccgagat ctcaatcgcg ctgaaaatgg cctcagcaga gcccactcgt 181 caagtgagga gacatcgtca gtgctgcagc cggggatcgc catggagacc agaggactgg 241 ctgactccgg gcagggctcc ttcaccggcc aggggatcgc caggctgtcg cgcctcatct 301 tcttgctgcg caggtgggct gccaggcatg tgcaccacca ggaccaggga ccggactctt 361 ttcctgatcg tttccgtgga gccgagctta aggaggtgtc cagccaagaa agcaatgccc 421 aggcaaatgt gggcagccag gagccagcag acagagggag aagcgcctgg cccctggcca 481 aatgcaacac taacaccagc aacaacacgg aggaggagaa gaagacgaaa aagaaggatg 541 cgatcgtggt ggacccgtcc agcaacctgt actaccgctg gctgaccgcc atcgccctgc 601 ctgtcttcta taactggtat ctgcttattt gcagggcctg tttcgatgag ctgcagtccg 661 agtacctgat gctgtggctg gtcctggact actcggcaga tgtcctgtat gtcttggatg 721 tgcttgtacg agctcggaca ggttttctcg agcaaggctt aatggtcagt gataccaaca 781 ggctgtggca gcattacaag acgaccacgc agttcaagct ggatgtgttg tccctggtcc 841 ccaccgacct ggcttactta aaggtgggca caaactaccc agaagtgagg ttcaaccgcc 901 tactgaagtt ttcccggctc tttgaattct ttgaccgcac agagacaagg accaactacc 961 ccaatatgtt caggattggg aacttggtct tgtacattct catcatcatc cactggaatg 1021 cctgcatcta ctttgccatt tccaagttca ttggttttgg gacagactcc tgggtctacc 1081 caaacatctc aatcccagag catgggcgcc tctccaggaa gtacatttac agtctctact 1141 ggtccacctt gacccttacc accattggtg agaccccacc ccccgtgaaa gatgaggagt 1201 atctctttgt ggtcgtagac ttcttggtgg gtgttctgat ttttgccacc attgtgggca 1261 atgtgggctc catgatctcg aatatgaatg cctcacgggc agagttccag gccaagattg 1321 attccatcaa gcagtacatg cagttccgca aggtcaccaa ggacttggag acgcgggtta 1381 tccggtggtt tgactacctg tgggccaaca agaagacggt ggatgagaag gaggtgctca 1441 agagcctccc agacaagctg aaggctgaga tcgccatcaa cgtgcacctg gacacgctga 1501 agaaggttcg catcttccag gactgtgagg cagggctgct ggtggagctg gtgctgaagc 1561 tgcgacccac tgtgttcagc cctggggatt atatctgcaa gaagggagat attgggaagg 1621 agatgtacat catcaacgag ggcaagctgg ccgtggtggc tgatgatggg gtcacccagt 1681 tcgtggtcct cagcgatggc agctacttcg gggagatcag cattctgaac atcaagggga 1741 gcaagtcggg gaaccgcagg acggccaaca tccgcagcat tggctactca gacctgttct 1801 gcctctcaaa ggacgatctc atggaggccc TCACCGAGTA CCCCGAAGCC AAGAAGGCCC 1861 TGGAGGAGAA AGGACGGCAG ATCCTGATGA AAGACAACCT GATCGATGAG GAGCTGGCCA 1921 GGGCGGGCGC GGACCCCAAG GACCTTGAGG AGAAAGTGGA GCAGCTGGGG TCCTCCCTGG 1981 ACACCCTGCA GACCAGGTTT GCACGCCTCC TGGCTGAGTA CAACGCCACC CAGATGAAGA 2041 TGAAGCAGCG TCTCAGCCAA CTGGAAAGCC AGGTGAAGGG TGGTGGGGAC AAGCCCCTGG 2101 CTGATGGGGA AGTTCCCGGG GATGCTACAA AAACAGAGGA CAAACAACAG TTGCCAACTT 2161 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 2221 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 2281 CTTGTGGAAA GGACGACAGT AAACTGGAAC ATCCGATTTC ACGCGTTAAG TCgacaatca 2341 acctctggat tacaaaattt gtgaaagatt