Construct: ORF TRCN0000467967
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015391.1_s317c1
- Derived from:
- ccsbBroadEn_06046
- DNA Barcode:
- ACCCATGAACGGTCAAGAAATGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CRYGD (1421)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467967
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1421 | CRYGD | crystallin gamma D | NM_006891.4 | 99.6% | 100% | 51T>C;285A>G |
| 2 | mouse | 12967 | Crygd | crystallin, gamma D | NM_007776.2 | 88.5% | 84.4% | (many diffs) |
| 3 | mouse | 12968 | Cryge | crystallin, gamma E | NM_007777.3 | 86.8% | 79.8% | (many diffs) |
| 4 | mouse | 12969 | Crygf | crystallin, gamma F | NM_027010.2 | 86.5% | 79.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 591
- ORF length:
- 522
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggaagatc accctctacg aggaccgggg cttccagggc cgccactacg 121 aatgcagcag cgaccacccc aacctgcagc cctacttgag ccgctgcaac tcggcgcgcg 181 tggacagcgg ctgctggatg ctctatgagc agcccaacta ctcgggcctc cagtacttcc 241 TGCGCCGCGG CGACTATGCC GACCACCAGC AGTGGATGGG CCTCAGCGAC TCGGTCCGCT 301 CCTGCCGCCT CATCCCCCAC TCTGGCTCTC ACAGGATCAG ACTCTATGAG AGGGAGGACT 361 ACAGAGGCCA GATGATAGAG TTCACTGAGG ACTGCTCCTG TCTTCAGGAC CGCTTCCGCT 421 TCAATGAAAT CCACTCCCTC AACGTGCTGG AGGGCTCCTG GGTCCTCTAC GAGCTGTCCA 481 ACTACCGAGG ACGGCAGTAC CTGCTGATGC CAGGGGACTA TAGGCGCTAC CAGGACTGGG 541 GGGCCACGAA TGCCAGAGTG GGCTCTCTGA GGAGAGTCAT AGATTTCTCC TTGCCAACTT 601 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 661 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 721 CTTGTGGAAA GGACGAACCC ATGAACGGTC AAGAAATGTT ACGCGTTAAG TCgacaatca 781 acctctggat tacaaaattt gtgaaagatt