Construct: ORF TRCN0000467984
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017181.1_s317c1
- Derived from:
- ccsbBroadEn_08976
- DNA Barcode:
- GCACCTTAATTGCGAAAGGTTCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BAALC (79870)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467984
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 79870 | BAALC | BAALC binder of MAP3K1 and ... | NM_024812.3 | 99.5% | 99.3% | 12C>T;425A>C |
2 | human | 79870 | BAALC | BAALC binder of MAP3K1 and ... | NM_001364874.1 | 80.1% | 80% | 12C>T;160_264del;530A>C |
3 | human | 79870 | BAALC | BAALC binder of MAP3K1 and ... | NM_001024372.2 | 37% | 37.2% | 12C>T;162_163ins273 |
4 | human | 79870 | BAALC | BAALC binder of MAP3K1 and ... | XR_001745601.2 | 22.2% | (many diffs) | |
5 | mouse | 118452 | Baalc | brain and acute leukemia, c... | NM_080640.5 | 86.2% | 82.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 501
- ORF length:
- 435
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ctgcggtggg agccgggcgg atgccatcga gccccgctac tacgagagct 121 ggacccggga gacagaatcc acctggctca cctacaccga cTCGGACGCG CCGCCCAGCG 181 CCGCCGCCCC GGACAGCGGC CCCGAAGCGG GCGGCCTGCA CTCGGGCATG CTGGAAGATG 241 GACTGCCCTC CAATGGTGTG CCCCGATCTA CAGCCCCAGG TGGAATACCC AACCCAGAGA 301 AGAAGACGAA CTGTGAGACC CAGTGCCCAA ATCCCCAGAG CCTCAGCTCA GGCCCTCTGA 361 CCCAGAAACA GAATGGCCTT CAGACCACAG AGGCTAAAAG AGATGCTAAG AGAATGCCTG 421 CAAAAGAAGT CACCATTAAT GTAACAGATA GCATCCAACA GATGGACAGA AGTCGAAGAA 481 TCACAAAGAC CTGTGTCAAC TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 541 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 601 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCAC CTTAATTGCG 661 AAAGGTTCAG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt