Construct: ORF TRCN0000468013

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007792.1_s317c1
Derived from:
ccsbBroadEn_13003
DNA Barcode:
GACAAGAAGATGACTTCTGCTATT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SFXN1 (94081)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468013
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 94081 SFXN1 sideroflexin 1 NM_001322978.2 90.4% 90.4% 1_75del
2 human 94081 SFXN1 sideroflexin 1 NM_001322982.2 90.4% 90.4% 1_75del
3 human 94081 SFXN1 sideroflexin 1 NM_001322977.2 73.2% 73.2% 1_258del
4 human 94081 SFXN1 sideroflexin 1 NM_022754.7 73.2% 73.2% 1_258del
5 human 94081 SFXN1 sideroflexin 1 NM_001322980.2 54.3% 38.1% 1_258del;595_596ins128;783_784ins55
6 human 94081 SFXN1 sideroflexin 1 NM_001322981.2 54.3% 38.1% 1_258del;595_596ins128;783_784ins55
7 human 94081 SFXN1 sideroflexin 1 NM_001322983.2 48.2% 48.1% 1_258del;725_726delGTins242
8 mouse 14057 Sfxn1 sideroflexin 1 NM_027324.5 62.7% 69.8% (many diffs)
9 mouse 14057 Sfxn1 sideroflexin 1 XM_006516849.3 62.7% 69.8% (many diffs)
10 mouse 14057 Sfxn1 sideroflexin 1 XM_006516850.3 62.7% 69.8% (many diffs)
11 mouse 14057 Sfxn1 sideroflexin 1 XM_011244373.2 47.7% 51.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
774
ORF length:
708
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat tttgatagga agaatgtcag cccaggttcc catgaacatg accatcacag
121gttgtatgat gacgttttac aggactacgc cggctgtgct gttctggcag tggattaacc
181agtccttcaa tgccgtcgtc aattacacca acagaagtgg agacgcaccc ctcactgtca
241atgagttggg aacagcttac gtttctgcaa caactggtgc cGTAGCAACA GCTCTAGGAC
301TCAATGCATT GACCAAGCAT GTCTCACCAC TGATAGGACG TTTTGTTCCC TTTGCTGCCG
361TAGCTGCTGC TAATTGCATT AATATTCCAT TAATGAGGCA AAGGGAACTC AAAGTTGGCA
421TTCCCGTCAC GGATGAGAAT GGGAACCGCT TGGGGGAGTC GGCGAACGCT GCGAAACAAG
481CCATCACGCA AGTTGTCGTG TCCAGGATTC TCATGGCAGC CCCTGGCATG GCCATCCCTC
541CATTCATTAT GAACACTTTG GAAAAGAAAG CCTTTTTGAA GAGGTTCCCA TGGATGAGTG
601CACCCATTCA AGTTGGGTTA GTTGGCTTCT GTTTGGTGTT TGCTACACCC CTGTGTTGTG
661CCCTGTTTCC TCAGAAAAGT TCCATGTCTG TGACAAGCTT GGAGGCCGAG TTGCAAGCTA
721AGATCCAAGA GAGCCATCCT GAATTGCGAC GCGTGTACTT CAATAAGGGA TTGTACCCAA
781CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG
841ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA
901TATCTTGTGG AAAGGACGAG ACAAGAAGAT GACTTCTGCT ATTACGCGTT AAGTCgacaa
961tcaacctctg gattacaaaa tttgtgaaag att

Download FASTA (ORF) (Full)