Construct: ORF TRCN0000468013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007792.1_s317c1
- Derived from:
- ccsbBroadEn_13003
- DNA Barcode:
- GACAAGAAGATGACTTCTGCTATT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SFXN1 (94081)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468013
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 94081 | SFXN1 | sideroflexin 1 | NM_001322978.2 | 90.4% | 90.4% | 1_75del |
| 2 | human | 94081 | SFXN1 | sideroflexin 1 | NM_001322982.2 | 90.4% | 90.4% | 1_75del |
| 3 | human | 94081 | SFXN1 | sideroflexin 1 | NM_001322977.2 | 73.2% | 73.2% | 1_258del |
| 4 | human | 94081 | SFXN1 | sideroflexin 1 | NM_022754.7 | 73.2% | 73.2% | 1_258del |
| 5 | human | 94081 | SFXN1 | sideroflexin 1 | NM_001322980.2 | 54.3% | 38.1% | 1_258del;595_596ins128;783_784ins55 |
| 6 | human | 94081 | SFXN1 | sideroflexin 1 | NM_001322981.2 | 54.3% | 38.1% | 1_258del;595_596ins128;783_784ins55 |
| 7 | human | 94081 | SFXN1 | sideroflexin 1 | NM_001322983.2 | 48.2% | 48.1% | 1_258del;725_726delGTins242 |
| 8 | mouse | 14057 | Sfxn1 | sideroflexin 1 | NM_027324.5 | 62.7% | 69.8% | (many diffs) |
| 9 | mouse | 14057 | Sfxn1 | sideroflexin 1 | XM_006516849.3 | 62.7% | 69.8% | (many diffs) |
| 10 | mouse | 14057 | Sfxn1 | sideroflexin 1 | XM_006516850.3 | 62.7% | 69.8% | (many diffs) |
| 11 | mouse | 14057 | Sfxn1 | sideroflexin 1 | XM_011244373.2 | 47.7% | 51.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 774
- ORF length:
- 708
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tttgatagga agaatgtcag cccaggttcc catgaacatg accatcacag 121 gttgtatgat gacgttttac aggactacgc cggctgtgct gttctggcag tggattaacc 181 agtccttcaa tgccgtcgtc aattacacca acagaagtgg agacgcaccc ctcactgtca 241 atgagttggg aacagcttac gtttctgcaa caactggtgc cGTAGCAACA GCTCTAGGAC 301 TCAATGCATT GACCAAGCAT GTCTCACCAC TGATAGGACG TTTTGTTCCC TTTGCTGCCG 361 TAGCTGCTGC TAATTGCATT AATATTCCAT TAATGAGGCA AAGGGAACTC AAAGTTGGCA 421 TTCCCGTCAC GGATGAGAAT GGGAACCGCT TGGGGGAGTC GGCGAACGCT GCGAAACAAG 481 CCATCACGCA AGTTGTCGTG TCCAGGATTC TCATGGCAGC CCCTGGCATG GCCATCCCTC 541 CATTCATTAT GAACACTTTG GAAAAGAAAG CCTTTTTGAA GAGGTTCCCA TGGATGAGTG 601 CACCCATTCA AGTTGGGTTA GTTGGCTTCT GTTTGGTGTT TGCTACACCC CTGTGTTGTG 661 CCCTGTTTCC TCAGAAAAGT TCCATGTCTG TGACAAGCTT GGAGGCCGAG TTGCAAGCTA 721 AGATCCAAGA GAGCCATCCT GAATTGCGAC GCGTGTACTT CAATAAGGGA TTGTACCCAA 781 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 841 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 901 TATCTTGTGG AAAGGACGAG ACAAGAAGAT GACTTCTGCT ATTACGCGTT AAGTCgacaa 961 tcaacctctg gattacaaaa tttgtgaaag att