Construct: ORF TRCN0000468035
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005337.1_s317c1
- Derived from:
- ccsbBroadEn_00345
- DNA Barcode:
- CAGACTTTCTCATAGATTAGGAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC31A1 (1317)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468035
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1317 | SLC31A1 | solute carrier family 31 me... | NM_001859.4 | 100% | 100% | |
| 2 | mouse | 20529 | Slc31a1 | solute carrier family 31, m... | NM_175090.4 | 86.8% | 88.3% | (many diffs) |
| 3 | mouse | 20529 | Slc31a1 | solute carrier family 31, m... | XM_006537711.2 | 86.8% | 88.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tcattcccac catatgggga tgagctatat ggactccaac agtaccatgc 121 aaccttctca ccatcaccca accacttcag cctcacactc ccatggtgga ggagacagca 181 gcatgatgat gatgcctatg accttctact ttggctttaa gaatgtggaa cTACTGTTTT 241 CCGGTTTGGT GATCAATACA GCTGGAGAAA TGGCTGGAGC TTTTGTGGCA GTGTTTTTAC 301 TAGCAATGTT CTATGAAGGA CTCAAGATAG CCCGAGAGAG CCTGCTGCGT AAGTCACAAG 361 TCAGCATTCG CTACAATTCC ATGCCTGTCC CAGGACCAAA TGGAACCATC CTTATGGAGA 421 CACACAAAAC TGTTGGGCAA CAGATGCTGA GCTTTCCTCA CCTCCTGCAA ACAGTGCTGC 481 ACATCATCCA GGTGGTCATA AGCTACTTCC TCATGCTCAT CTTCATGACC TACAACGGGT 541 ACCTCTGCAT TGCAGTAGCA GCAGGGGCCG GTACAGGATA CTTCCTCTTC AGCTGGAAGA 601 AGGCAGTGGT AGTGGATATC ACAGAGCATT GCCATTGCCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 ACAGACTTTC TCATAGATTA GGAATACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt