Construct: ORF TRCN0000468041
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011482.1_s317c1
- Derived from:
- ccsbBroadEn_12733
- DNA Barcode:
- GGGCTATTTTATTCCAACGTCGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- QTRT1 (81890)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468041
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81890 | QTRT1 | queuine tRNA-ribosyltransfe... | NM_031209.3 | 88.8% | 88.8% | 1_135del |
| 2 | mouse | 60507 | Qtrt1 | queuine tRNA-ribosyltransfe... | NM_021888.2 | 74.6% | 77.9% | (many diffs) |
| 3 | mouse | 60507 | Qtrt1 | queuine tRNA-ribosyltransfe... | XM_006510524.3 | 54.2% | 56% | (many diffs) |
| 4 | mouse | 60507 | Qtrt1 | queuine tRNA-ribosyltransfe... | XM_006510525.2 | 47.3% | 49.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1140
- ORF length:
- 1074
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc agtgggcacg caggccacca tgaagggcat cacgaccgaa cagctggacg 121 ctctgggttg ccgcatctgc ctgggcaata cctaccatct gggtctaagg ccgggacccg 181 agctgatcca gaaagccaac ggtctccacg gcttcatgaa ttggcctcat aatctgctaa 241 cggacagcgg cggtttccag atggtgtcgc tggtgtctct gtccgaggtg acggaggagg 301 gcgtccgctt ccgctccccc tacgacggca atgagaccct gctgagcccg gagaaatccg 361 tgcagatcca gaatgcgctg ggctcggaca tcatcatgca gctggacgac gtggttagca 421 gtactgtgac tgggccacgt gtggaggagg ccatgtacag gtcaatccgc tggctggacc 481 ggtgcattgc agcccatcag cggccggaca agcagaacct cttcgccatt atccagggtg 541 ggctggacgc agatctccgg gccacctgcc ttgaagagat gaccaagcga gacgtgcctg 601 gcttcgccat cgggggcctg agcgggggtg agagcaagtc gcagttctgg cggatggtgg 661 cgctgagcac ctctcggctg ccgaaggaca agccccgata tctgatgggg gttGGCTATG 721 CCACTGATCT GGTAGTCTGC GTGGCTCTTG GATGTGACAT GTTCGACTGC GTCTTCCCCA 781 CACGGACAGC GCGCTTTGGC TCTGCCCTGG TGCCCACTGG GAACCTGCAG TTGAGGAAGA 841 AGGTGTTTGA GAAGGACTTC GGCCCCATAG ACCCGGAGTG CACCTGCCCC ACGTGCCAAA 901 AGCACAGCCG CGCCTTCCTG CACGCACTGC TGCACAGTGA CAACACGGCC GCGCTGCACC 961 ACCTCACGGT CCACAACATC GCCTACCAGC TGCAGCTCAT GAGCGCCGTC CGCACCAGCA 1021 TCGTGGAGAA GCGCTTCCCG GACTTCGTGC GGGACTTCAT GGGCGCCATG TACGGGGATC 1081 CCACCCTCTG TCCCACCTGG GCCACTGACG CTCTGGCCTC TGTGGGAATC ACACTGGGCT 1141 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1201 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1261 TTTATATATC TTGTGGAAAG GACGAGGGCT ATTTTATTCC AACGTCGGTA CGCGTTAAGT 1321 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt