Construct: ORF TRCN0000468082
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009330.1_s317c1
- Derived from:
- ccsbBroadEn_14342
- DNA Barcode:
- CCTGTGTACGAATGTCTCTTCTAT
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DPH7 (92715)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468082
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346393.2 | 81.7% | 75.6% | (many diffs) |
| 2 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346394.2 | 81.7% | 75.6% | (many diffs) |
| 3 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346395.2 | 81.7% | 75.6% | (many diffs) |
| 4 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346396.2 | 81.7% | 75.6% | (many diffs) |
| 5 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346375.2 | 76.4% | 68.9% | (many diffs) |
| 6 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346376.2 | 76.4% | 68.9% | (many diffs) |
| 7 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346377.2 | 76.4% | 68.9% | (many diffs) |
| 8 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346378.2 | 76.4% | 68.9% | (many diffs) |
| 9 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346379.2 | 76.4% | 68.9% | (many diffs) |
| 10 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346380.2 | 76.4% | 68.9% | (many diffs) |
| 11 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346381.2 | 76.4% | 68.9% | (many diffs) |
| 12 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346382.2 | 76.4% | 68.9% | (many diffs) |
| 13 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346383.2 | 76.4% | 68.9% | (many diffs) |
| 14 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346384.2 | 76.4% | 68.9% | (many diffs) |
| 15 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346385.2 | 76.4% | 68.9% | (many diffs) |
| 16 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346386.2 | 76.4% | 68.9% | (many diffs) |
| 17 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346387.2 | 76.4% | 68.9% | (many diffs) |
| 18 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346388.2 | 76.4% | 68.9% | (many diffs) |
| 19 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346389.2 | 76.4% | 68.9% | (many diffs) |
| 20 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346390.2 | 76.4% | 68.9% | (many diffs) |
| 21 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346391.2 | 76.4% | 68.9% | (many diffs) |
| 22 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346392.2 | 76.4% | 68.9% | (many diffs) |
| 23 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346373.2 | 66.5% | 60.2% | (many diffs) |
| 24 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_001346370.2 | 50.2% | 45.3% | (many diffs) |
| 25 | human | 92715 | DPH7 | diphthamide biosynthesis 7 | NM_138778.5 | 47% | 42.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg acaggtctct gggagacgtt ctgtgccccc caggggcctt gtggaacagg 121 tgagcctggc tttccttata tgcctcctgt ggttggtcgg tttcagctat gatgaacaca 181 tcctactgtg ggacacacga aacatgaagc agccgttggc agatacgcct gtgcagggtg 241 gggtatggag aatcaagtgg caccctttcc accacaacct gctcctggcc gcctgcatgc 301 acagtggctt taagatcctc aactgccaaa aggcaatgga ggagaggcag gaggcgacgg 361 TCCTGACATC TCACACATTG CCCGACTCGC TGGTGTATGG AGCCGACTGG TCCTGGCTGC 421 TCTTCCGTTC TCTGCAGCGG GCCCCCTCGT GGTCCTTTCC TAGCAACCTA GGAACCAAGA 481 CGGCAGACCT GAAGGGTGCA AGCGAGTTGC CAACACCCTG TCATGAATGC AGAGAGGATA 541 ACGATGGGGA GGGCCATGCC AGACCCCAGA GTGGAATGAA GCCACTCACA GAGGGCATGA 601 GGAAGAATGG CACCTGGCTG CAGGCTACAG CAGCCACCAC ACGTGACTGT GGCGTGAACC 661 CAGAAGAAGC AGACTCAGCC TTCAGCCTCC TGGCCACCTG CTCATTCTAT GACCATGCGC 721 TCCACCTCTG GGAGTGGGAG GGGACTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG 781 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 841 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCTGTGTAC 901 GAATGTCTCT TCTATACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 961 agatt