Construct: ORF TRCN0000468083
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007922.1_s317c1
- Derived from:
- ccsbBroadEn_11293
- DNA Barcode:
- GTTGCCGCCCGCTGCGTATAACAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PEA15 (8682)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468083
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8682 | PEA15 | proliferation and apoptosis... | NR_123724.2 | 9.8% | 1_1489del;1712_2246del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 182
- ORF end:
- 404
- ORF length:
- 222
- Sequence:
-
1 GTGGAGACTG AAGTTAGGCC AGCTTGGCAC TTGATGTAAT TCTCCTTGGA ATTTGCCCTT 61 TTTGAGTTTG GATCTTGGTT CATTCTCAAG CCTCAGACAG TGGTTCAAAG TTTTTTTCTT 121 CCATTTCAGG TGTCGTGAGG CTAGCATCGA TTGATCAACA AGTTTGTACA AAAAAGTTGG 181 CATGTATATT AAAACTGCAC TGCCATGTCT GCCCTTTTTT GTGGTGTCTA GCATTAACTT 241 ATTGTCTAGG CCAGAGCGGG GGTGGGAGGG GAATGCCACA GTGAAGGGAG TGGCAGAATC 301 AAATTGCTAC ATAGTCCAAA CAAAAAAGAA GGCTTTTTCA AAAAACATTA AATTCACATG 361 CAGTCTCAGA GACTATTTAG ACAAAGTTCA AGTTAGGAGC TTTTACCCAA CTTTCTTGTA 421 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 481 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 541 AAAGGACGAG TTGCCGCCCG CTGCGTATAA CAGACGCGTT AAGTCgacaa tcaacctctg 601 gattacaaaa tttgtgaaag att