Construct: ORF TRCN0000468088
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000588.1_s317c1
- Derived from:
- ccsbBroadEn_10064
- DNA Barcode:
- CGAGGACATCAGGCACGATTTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFS7 (374291)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468088
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374291 | NDUFS7 | NADH:ubiquinone oxidoreduct... | NM_024407.5 | 99.8% | 99.5% | 68C>T |
| 2 | human | 374291 | NDUFS7 | NADH:ubiquinone oxidoreduct... | NM_001363602.2 | 85.1% | 84.5% | 68C>T;545A>G;546_547ins93 |
| 3 | human | 374291 | NDUFS7 | NADH:ubiquinone oxidoreduct... | XM_024451499.1 | 83.9% | 80% | (many diffs) |
| 4 | mouse | 75406 | Ndufs7 | NADH:ubiquinone oxidoreduct... | NM_029272.4 | 77.3% | 81.4% | (many diffs) |
| 5 | mouse | 75406 | Ndufs7 | NADH:ubiquinone oxidoreduct... | XM_030245344.1 | 77.3% | 81.4% | (many diffs) |
| 6 | mouse | 75406 | Ndufs7 | NADH:ubiquinone oxidoreduct... | XM_006514270.2 | 76.9% | 78% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 705
- ORF length:
- 639
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggtgctgtca gctcctggcc tgcgcggctt ccggatcctt ggtctgcgct 121 ccagcgtggg cctggctgtg caggcacgag gtgtccatca gagcgtggcc accgatggcc 181 caagcagcac ccagcctgcc ctgccaaagg ccagagccgt ggctcccaaa cccagcagcc 241 ggggcgagta tgtggtggcc aagctggatg acctcgtcaa ctgggcccgc cggagttctc 301 tgtggcccat gaccttcggc ctggcctgcT GCGCCGTGGA GATGATGCAC ATGGCAGCAC 361 CCCGCTACGA CATGGACCGC TTTGGCGTGG TCTTCCGCGC CAGCCCGCGC CAGTCCGACG 421 TCATGATCGT GGCCGGCACA CTCACCAACA AGATGGCCCC AGCGCTTCGC AAGGTCTACG 481 ACCAGATGCC GGAGCCGCGC TACGTGGTCT CCATGGGGAG CTGCGCCAAC GGAGGAGGCT 541 ACTACCACTA TTCCTACTCG GTGGTGAGGG GCTGCGACCG CATCGTGCCC GTGGACATCT 601 ACATCCCAGG CTGCCCACCT ACGGCCGAGG CCCTGCTCTA CGGCATCCTG CAGCTGCAGA 661 GGAAGATCAA GCGGGAGCGG AGGCTGCAGA TCTGGTACCG CAGGTACCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA CGAGGACATC AGGCACGATT TAACACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt