Construct: ORF TRCN0000468093
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001362.1_s317c1
- Derived from:
- ccsbBroadEn_01813
- DNA Barcode:
- AAGGCCATTTATGTTGAGAAGCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF177 (7730)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468093
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100529215 | ZNF559-ZNF177 | ZNF559-ZNF177 readthrough | NM_001172650.3 | 100% | 100% | |
| 2 | human | 7730 | ZNF177 | zinc finger protein 177 | NM_003451.3 | 100% | 100% | |
| 3 | human | 7730 | ZNF177 | zinc finger protein 177 | NM_001172651.1 | 66.7% | 66.7% | 336_815del |
| 4 | human | 100529215 | ZNF559-ZNF177 | ZNF559-ZNF177 readthrough | NM_001202425.1 | 37.2% | 36.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1029
- ORF length:
- 963
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgcagggtgg ctgacaacct ggtcacagaa ctcagtaacc ttccaggaag 121 tggcagtgga cttttcccag gaggagtggg cattgctgga ccctgctcaa aaaaatctat 181 acaaagatgt gatgctggag aactttagga acctggcctc agtagggtat cagctctgca 241 gacacagtct gatctccaag gtggatcaag aacagctgaa gacagatgaa agaggaattt 301 tacaaggtga ctgtgcagac tgggaaactc aacttaaacc aaaagataca attgctatgc 361 agaacattcc tgggggaaaa acatccaatg gcataaacac gaactgtgtc agaactcact 421 ctggagagat gccctatgaa tgcagtgact gtgggaaagc cttcattttt cagtcttccc 481 ttaagaaaca catgagatct catactggag agaagcctta tgagtgtgat cactgtggaa 541 aatcctttag ccagagctct catctgaatg tgcacaaaag aactcacact ggagagaaac 601 cctatgactg taaggaatgt gggaaggctt tcactgttcc ttcatcccTT CAGAAACATG 661 TGAGAACCCA CACTGGAGAG AAACCCTATG AATGCAGTGA CTGTGGAAAA GCCTTCATCG 721 ATCAGTCATC CCTTAAGAAA CACACACGCT CTCACACTGG AGAGAAGCCT TATGAGTGTA 781 ACCAGTGTGG AAAGTCCTTC AGCACAGGCT CTTACCTTAT TGTGCACAAG AGAACTCACA 841 CTGGTGAGAA AACCTATGAG TGTAAAGAAT GTGGGAAGGC CTTTAGGAAT TCCTCTTGCC 901 TGAGGGTACA CGTGAGAACT CACACTGGAG AGAAGCCTTA TAAATGTATT CAGTGTGAAA 961 AAGCCTTTAG CACAAGCACT AACCTTATAA TGCACAAGCG AATCCACAAT GGCCAGAAAC 1021 TCCATGAATG CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 1081 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 1141 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGAAAGGCC ATTTATGTTG AGAAGCCTAC 1201 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt