Construct: ORF TRCN0000468101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004400.1_s317c1
- Derived from:
- ccsbBroadEn_00232
- DNA Barcode:
- GCCTGTCTTCGATTATGCGCAGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SERPINA6 (866)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468101
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 866 | SERPINA6 | serpin family A member 6 | NM_001756.4 | 99.9% | 99.7% | 736T>G |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1281
- ORF length:
- 1215
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc actcctcctg tacacctgtc ttctctggct gcccaccagc ggcctctgga 121 ccgtccaggc catggatcct aacgctgctt atgtgaacat gagtaaccat caccggggcc 181 tggcttcagc caacgttgac tttgccttca gcctgtataa gcacctagtg gccttgagtc 241 ccaaaaagaa cattttcatc tcccctgtga gcatctccat ggccttagct atgctgtccc 301 tgggcacctg tggccacaca cgggcccagc ttctccaggg cctgggtttc aacctcactg 361 agaggtctga gactgagatc caccagggtt tccagcacct gcaccaactc tttgcaaagt 421 cagacaccag cttagaaatg accatgggca atgccttgtt tcttgatggc agcctggagt 481 tgctggagtc attctcagca gacatcaagc actactatga gtcagaggtc ttggctatga 541 atttccagga ctgggcaaca gccagcagac agatcaacag ctatgtcaag aataagacac 601 aggggaaaat tgtcgacttg ttttcagggc tggatagccc agccatcctc gtcctggtca 661 actatatctt cttcaaaggc acatggacac agccctttga cctggcaagc accagggagg 721 agaacttcta tgtggacgag acaactgtgg tgaaggtgcc catgatgttg cagtcgagca 781 ccatcagtta ccttcatgac gcggagctcc cctgccagct ggtgcagatg aactacgtgg 841 gcaatgggac tgtcttcttc atccttccgg acaaggggaa gatgaacaca gtcatcgctg 901 cactgagccg ggacacgatt aacaggtggT CCGCAGGCCT GACCAGCAGC CAGGTGGACC 961 TGTACATTCC AAAGGTCACC ATCTCTGGAG TCTATGACCT CGGAGATGTG CTGGAGGAAA 1021 TGGGCATTGC AGACTTGTTC ACCAACCAGG CAAATTTCTC ACGCATCACC CAGGACGCCC 1081 AGCTGAAGTC ATCAAAGGTG GTCCATAAAG CTGTGCTGCA ACTCAATGAG GAGGGTGTGG 1141 ACACAGCTGG CTCCACTGGG GTCACCCTAA ACCTGACGTC CAAGCCTATC ATCTTGCGTT 1201 TCAACCAGCC CTTCATCATC ATGATCTTCG ACCACTTCAC CTGGAGCAGC CTTTTCCTGG 1261 CGAGGGTTAT GAACCCAGTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1321 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1381 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGCCT GTCTTCGATT 1441 ATGCGCAGCA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt