Construct: ORF TRCN0000468103
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014479.1_s317c1
- Derived from:
- ccsbBroadEn_07596
- DNA Barcode:
- ATTCAACCGATCACGTAATGGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DLC1 (10395)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468103
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10395 | DLC1 | DLC1 Rho GTPase activating ... | NM_024767.4 | 99.9% | 99.7% | 763A>G |
| 2 | human | 10395 | DLC1 | DLC1 Rho GTPase activating ... | NM_001348081.2 | 30% | 29.5% | (many diffs) |
| 3 | human | 10395 | DLC1 | DLC1 Rho GTPase activating ... | NM_182643.3 | 30% | 29.5% | (many diffs) |
| 4 | human | 10395 | DLC1 | DLC1 Rho GTPase activating ... | XM_005273374.1 | 30% | 29.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1455
- ORF length:
- 1389
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtagctatc agaaagagaa gctgggaaga acatgtgacc cactggatgg 121 gacagccttt taattctgat gatcgtaaca cagcatgtca tcatggacta gtagctgaca 181 gcttgcaggc aagtatggaa aaagatgcaa ctctaaatgt ggaccgcaaa gagaagtgtg 241 tttcactacc tgactgctgt catggatcag agctgagaga ttttcctggg aggccaatgg 301 gtcatctttc aaaggatgtg gacgaaaatg acagccatga aggtgaagat cagtttcttt 361 ctctggaagc cagcacagaa acactagtgc atgtttctga tgaggataac aatgctgatt 421 tatgccttac agatgataaa caggttttaa atacccaagg gcagaaaaca tcaggccaac 481 atatgatcca aggagcaggc tccttagaaa aggcactgcc catcatacaa agtaaccaag 541 tttcttctaa ctcctgggga atagctggtg aaactgaatt agcactggta aaagaaagtg 601 gggagagaaa agttactgac tctataagta aaagcctgga gctttgcaat gaaataagct 661 taagtgaaat aaaagatgca cccaaagtaa atgcagtgga tactttgaac gtgaaagata 721 ttgcacctga gaaacaattg cttaactctg ctgtaattgc tcagcaacga aggaaacctg 781 acccccctaa agatgaaaat gaaagaagca cctgcaatgt agtacaagat gagttcttgg 841 atactccttg cacaaacaga ggactgccat tattaaaaac agattttgga agctgccttc 901 tgcagcctcc ttcctgcccc aatggaatgt cagctgaaaa tggcctggag aagagtggtt 961 tttcacaaca tcaaaacaaa agtccaccaa aggtcaaggc agaagatggc atgcagtgtt 1021 tacaattaaa ggagaccctg gccacccagg aacccacaga taaccaagtc agacTTCGTA 1081 AGAGAAAGGA AATAAGAGAA GATCGAGATA GGGCGCGGCT GGACTCCATG GTGCTGCTGA 1141 TTATGAAACT GGACCAGCTT GATCAGGACA TAGAAAATGC CCTCAGCACC AGCTCCTCTC 1201 CATCAGGCAC ACCAACAAAC CTGCGGCGGC ACGTTCCTGA TCTGGAATCA GGATCTGAAA 1261 GTGGAGCAGA TACCATTTCA GTAAATCAGA CACGAGTAAA TTTGTCTTCT GACACTGAGT 1321 CCACGGACCT CCCATCTTCC ACTCCAGTAG CCAATTCTGG AACCAAACCC AAGACTACGG 1381 CTATTCAAGG TATTTCAGAG AAGGAAAAGG CTGGTAAGTT GACATTTTGG TTCTGTTTTC 1441 TCGCCAATCT ATTTTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1501 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1561 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA ATTCAACCGA TCACGTAATG 1621 GTCGACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt