Construct: ORF TRCN0000468114
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002101.1_s317c1
- Derived from:
- ccsbBroadEn_06713
- DNA Barcode:
- ACCGATCTAACCTAGAGAAATGCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PFDN4 (5203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468114
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5203 | PFDN4 | prefoldin subunit 4 | NM_002623.4 | 99.7% | 100% | 177T>A |
| 2 | human | 5203 | PFDN4 | prefoldin subunit 4 | XM_017027879.1 | 72.7% | 70.1% | (many diffs) |
| 3 | mouse | 109054 | Pfdn4 | prefoldin 4 | NM_001110152.2 | 85.8% | 97% | (many diffs) |
| 4 | mouse | 109054 | Pfdn4 | prefoldin 4 | NM_001199902.1 | 83.3% | 94.7% | (many diffs) |
| 5 | mouse | 109054 | Pfdn4 | prefoldin 4 | NM_001013369.2 | 47.5% | 55.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttG TACAAAAAAG 61 TTGGCATGGC GGCCACCATG AAGAAGGCGG CTGCAGAAGA TGTCAATGTT ACTTTCGAAG 121 ATCAACAAAA GATAAACAAA TTTGCACGGA ATACAAGTAG AATCACAGAG CTGAAGGAAG 181 AAATAGAAGT AAAAAAGAAA CAACTCCAAA ACCTAGAAGA TGCTTGTGAT GACATCATGC 241 TAGCAGATGA TGATTGCTTA ATGATACCTT ATCAAATTGG TGATGTCTTC ATTAGCCATT 301 CTCAAGAAGA AACGCAAGAA ATGTTAGAAG AAGCAAAGAA AAATTTGCAA GAAGAAATTG 361 ACGCCTTAGA ATCCAGAGTG GAATCAATTC AGCGAGTGTT AGCAGATTTG AAAGTTCAGT 421 TGTATGCAAA ATTCGGGAGC AACATAAACC TTGAAGCTGA TGAAAGTTAC CCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAACCGATC TAACCTAGAG AAATGCAACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt