Construct: ORF TRCN0000468158
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007566.1_s317c1
- Derived from:
- ccsbBroadEn_03371
- DNA Barcode:
- TGTGCATACAATTGTAGATGAGGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB23 (51715)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468158
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51715 | RAB23 | RAB23, member RAS oncogene ... | NM_001278666.1 | 100% | 100% | |
| 2 | human | 51715 | RAB23 | RAB23, member RAS oncogene ... | NM_001278667.1 | 100% | 100% | |
| 3 | human | 51715 | RAB23 | RAB23, member RAS oncogene ... | NM_001278668.1 | 100% | 100% | |
| 4 | human | 51715 | RAB23 | RAB23, member RAS oncogene ... | NM_016277.5 | 100% | 100% | |
| 5 | human | 51715 | RAB23 | RAB23, member RAS oncogene ... | NM_183227.2 | 100% | 100% | |
| 6 | mouse | 19335 | Rab23 | RAB23, member RAS oncogene ... | NM_001159729.1 | 87% | 93.2% | (many diffs) |
| 7 | mouse | 19335 | Rab23 | RAB23, member RAS oncogene ... | NM_008999.4 | 87% | 93.2% | (many diffs) |
| 8 | mouse | 19335 | Rab23 | RAB23, member RAS oncogene ... | XM_006495801.3 | 87% | 93.2% | (many diffs) |
| 9 | mouse | 19335 | Rab23 | RAB23, member RAS oncogene ... | XM_006495802.3 | 87% | 93.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtt ggaggaagat atggaagtcg ccataaagat ggtggttgta gggaatggag 121 cagttggaaa atcaagtatg attcagcgat attgcaaagg catttttaca aaagactaca 181 agaaaaccat tggagttgat tttttggagc gacaaattca agttaatgat gaagatgtca 241 gactaatgtt atgggacact gcaggtcagg aggaatttga tgcaattaca aaggcctact 301 atcgaggagc ccaggcttgt gtgctcgtgt tctctaccac agataggGAA TCTTTTGAAG 361 CAGTTTCCAG TTGGAGAGAG AAAGTAGTAG CCGAAGTGGG AGATATACCA ACTGTACTTG 421 TGCAAAACAA GATTGATCTT CTGGATGATT CTTGTATAAA GAATGAGGAA GCTGAGGCAC 481 TGGCAAAAAG GTTAAAGTTA AGATTCTACA GAACATCAGT GAAAGAAGAT CTAAATGTGA 541 ATGAAGTTTT TAAGTATTTG GCTGAAAAAT ACCTTCAGAA ACTCAAACAA CAAATAGCTG 601 AGGATCCAGA ACTAACGCAT TCAAGTAGTA ACAAGATTGG TGTCTTTAAT ACATCTGGTG 661 GAAGTCACTC CGGTCAGAAT TCAGGTACCC TCAATGGTGG AGATGTCATC AATCTTAGAC 721 CCAACAAACA AAGGACCAAG AAAAACAGAA ATCCTTTTAG CAGCTGTAGC ATACCCTACC 781 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 841 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 901 ATATATCTTG TGGAAAGGAC GATGTGCATA CAATTGTAGA TGAGGCACGC GTTAAGTCga 961 caatcaacct ctggattaca aaatttgtga aagatt