Construct: ORF TRCN0000468190
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017769.1_s317c1
- Derived from:
- ccsbBroadEn_04628
- DNA Barcode:
- TAGCCGCTTAGAGCGAACATTCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAT2 (112483)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468190
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 112483 | SAT2 | spermidine/spermine N1-acet... | NM_133491.5 | 100% | 100% | |
| 2 | human | 112483 | SAT2 | spermidine/spermine N1-acet... | NM_001320847.2 | 80% | 80% | 200_201ins102 |
| 3 | human | 112483 | SAT2 | spermidine/spermine N1-acet... | NM_001320845.1 | 68.2% | 60.9% | 1_220del;286_302del |
| 4 | human | 112483 | SAT2 | spermidine/spermine N1-acet... | NM_001320846.1 | 54.6% | 47.4% | 1_220del;286_302del;437_438ins102 |
| 5 | human | 112483 | SAT2 | spermidine/spermine N1-acet... | XM_017024073.1 | 49.3% | 40.6% | (many diffs) |
| 6 | human | 112483 | SAT2 | spermidine/spermine N1-acet... | XM_017024074.1 | 35.7% | 27% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 576
- ORF length:
- 510
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ttccgtgcgg atccgagagg ccaaggaggg agactgtgga gatatcctga 121 ggctgattcg ggagctagcc gaattcgaaa aactctcgga tcaggtgaag atcagtgaag 181 aagccctgag agcagatggc tttggagaca atcctTTCTA TCACTGTTTG GTAGCAGAGA 241 TTCTTCCAGC GCCCGGGAAG CTACTGGGGC CCTGCGTGGT GGGCTATGGG ATATACTATT 301 TCATCTACAG TACATGGAAG GGACGCACCA TTTATCTGGA GGATATCTAT GTGATGCCGG 361 AATATCGGGG TCAAGGGATT GGTTCCAAAA TAATCAAAAA GGTGGCTGAG GTGGCCTTGG 421 ATAAGGGCTG CTCCCAATTC CGCCTGGCCG TCCTGGACTG GAACCAGAGG GCCATGGACT 481 TGTACAAGGC CCTAGGAGCC CAAGATCTGA CGGAAGCTGA GGGCTGGCAC TTCTTCTGCT 541 TTCAAGGAGA GGCAACGAGA AAGTTGGCAG GAAAGTGCCC AACTTTCTTG TACAAAGTGG 601 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 661 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 721 ATAGCCGCTT AGAGCGAACA TTCCTACGCG TTAAGTCgac aatcaacctc tggattacaa 781 aatttgtgaa agatt