Construct: ORF TRCN0000468206
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014059.1_s317c1
- Derived from:
- ccsbBroadEn_00584
- DNA Barcode:
- CCTGTATACAGTACGATAGAGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FMOD (2331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468206
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2331 | FMOD | fibromodulin | NM_002023.5 | 100% | 100% | |
| 2 | mouse | 14264 | Fmod | fibromodulin | NM_021355.3 | 88.7% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1194
- ORF length:
- 1128
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gtggacctcc ctcctgctgc tggcagggct cttctccctc tcccaggccc 121 agtatgaaga tgaccctcat tggtggttcc actacctccg cagccagcag tccacctact 181 acgatcccta tgacccttac ccgtatgaga cctacgagcc ttacccctat ggggtggatg 241 aagggccagc ctacacctac ggctctccat cccctccaga tccccgcgac tgcccccagg 301 agtgcgactg cccacccaac ttccccacgg ccatgtactg tgacaatcgc aacctcaagt 361 acctgccctt cgttccctcc cgcatgaagt atgtgtactt ccagaacaac cagatcacct 421 ccatccagga aggcgtcttt gacaatgcca cagggctgct ctggattgct ctccacggca 481 accagatcac cagtgataag gtgggcagga aggtcttctc caagctgagg cacctggaga 541 ggctgtacct ggaccacaac aacctgaccc ggatgcccgg tcccctgcct cgatccctga 601 gagagctcca tctcgaccac aaccagatct cacgggtccc caacaatgct ctggaggggc 661 tggagaacct cacggccttg tacctccaac acaatgagat ccaggaagtg ggcagttcca 721 tgaggggcct ccggtcactg atcttgctgg accTGAGTTA TAACCACCTT CGGAAGGTGC 781 CTGATGGGCT GCCCTCAGCT CTTGAGCAGC TGTACATGGA GCACAACAAT GTCTACACCG 841 TCCCCGATAG CTACTTCCGG GGGGCGCCCA AGCTGCTGTA TGTGCGGCTG TCCCACAACA 901 GTCTAACCAA CAATGGCCTG GCCTCCAACA CCTTCAATTC CAGCAGCCTC CTTGAGCTAG 961 ACCTCTCCTA CAACCAGCTG CAGAAGATCC CCCCAGTCAA CACCAACCTG GAGAACCTCT 1021 ACCTCCAAGG CAATAGGATC AATGAGTTCT CCATCAGCAG CTTCTGCACC GTGGTGGACG 1081 TCGTGAACTT CTCCAAGCTG CAGGTGCTGC GCCTGGACGG GAACGAGATC AAGCGCAGCG 1141 CCATGCCTGC CGACGCGCCC CTCTGCCTGC GCCTTGCCAG CCTCATCGAG ATCTGCCCAA 1201 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 1261 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 1321 TATCTTGTGG AAAGGACGAC CTGTATACAG TACGATAGAG ACCACGCGTT AAGTCgacaa 1381 tcaacctctg gattacaaaa tttgtgaaag att