Construct: ORF TRCN0000468210
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008002.1_s317c1
- Derived from:
- ccsbBroadEn_01329
- DNA Barcode:
- AAAACCTTCTTTATGTACGACGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTBP1 (5725)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468210
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | NM_031991.4 | 100% | 100% | |
| 2 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XM_005259598.2 | 99.4% | 99% | 3_8delGCGTGC;11G>A;13A>G |
| 3 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | NM_031990.4 | 96.5% | 96.5% | 892_948del |
| 4 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | NM_002819.5 | 95.3% | 95.3% | 892_969del |
| 5 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XM_005259597.2 | 94.8% | 94.4% | (many diffs) |
| 6 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XR_244035.2 | 42.1% | (many diffs) | |
| 7 | human | 5725 | PTBP1 | polypyrimidine tract bindin... | XR_244034.2 | 41.2% | (many diffs) | |
| 8 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | NM_008956.3 | 86.8% | 96.9% | (many diffs) |
| 9 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | NM_001077363.2 | 82.7% | 92.4% | (many diffs) |
| 10 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | NM_001283013.1 | 80.7% | 90% | (many diffs) |
| 11 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | XR_380379.1 | 62.9% | (many diffs) | |
| 12 | mouse | 19205 | Ptbp1 | polypyrimidine tract bindin... | XR_001779485.1 | 41.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1659
- ORF length:
- 1593
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cggcattgtc ccagatatag ccgttggtac aaagcgggga tctgacgagc 121 ttttctctac ttgtgtcact aacggaccgt ttatcatgag cagcaactcg gcttctgcag 181 caaacggaaa tgacagcaag aagttcaaag gtgacagccg aagtgcaggc gtcccctcta 241 gagtgatcca catccggaag ctccccatcg acgtcacgga gggggaagtc atctccctgg 301 ggctgccctt tgggaaggtc accaacctcc tgatgctgaa ggggaaaaac caggccttca 361 tcgagatgaa cacggaggag gctgccaaca ccatggtgaa ctactacacc tcggtgaccc 421 ctgtgctgcg cggccagccc atctacatcc agttctccaa ccacaaggag ctgaagaccg 481 acagctctcc caaccaggcg cgggcccagg cggccctgca ggcggtgaac tcggtccagt 541 cggggaacct ggccttggct gcctcggcgg cggccgtgga cgcagggatg gcgatggccg 601 ggcagagccc cgtgctcagg atcatcgtgg agaacctctt ctaccctgtg accctggatg 661 tgctgcacca gattttctcc aagttcggca cagtgttgaa gatcatcacc ttcaccaaga 721 acaaccagtt ccaggccctg ctgcagtatg cggaccccgt gagcgcccag cacgccaagc 781 tgtcgctgga cgggcagaac atctacaacg cctgctgcac gctgcgcatc gacttttcca 841 agctcaccag cctcaacgtc aagtacaaca atgacaagag ccgtgactac acacgcccag 901 acctgccttc cggggacagc cagccctcgc tggaccagac catggccgcg gccttcggcc 961 tttccgttcc gaacgtccac ggcgccctgg cccccctggc catcccctcg gcggcggcgg 1021 cagctgcggc ggcaggtcgg atcgccatcc cgggcctggc gggggcagga aattctgtat 1081 tgctggtcag caacctcaac ccagagagag tcacacccca aagcctcttt attcttttcg 1141 gcgtctacgg tgacgtgcag cgcgtgaaga tcctgttcaa taagaaggag aacgcccTAG 1201 TGCAGATGGC GGACGGCAAC CAGGCCCAGC TGGCCATGAG CCACCTGAAC GGGCACAAGC 1261 TGCACGGGAA GCCCATCCGC ATCACGCTCT CGAAGCACCA GAACGTGCAG CTGCCCCGCG 1321 AGGGCCAGGA GGACCAGGGC CTGACCAAGG ACTACGGCAA CTCACCCCTG CACCGCTTCA 1381 AGAAGCCGGG CTCCAAGAAC TTCCAGAACA TATTCCCGCC CTCGGCCACG CTGCACCTCT 1441 CCAACATCCC GCCCTCAGTC TCCGAGGAGG ATCTCAAGGT CCTGTTTTCC AGCAATGGGG 1501 GCGTCGTCAA AGGATTCAAG TTCTTCCAGA AGGACCGCAA GATGGCACTG ATCCAGATGG 1561 GCTCCGTGGA GGAGGCGGTC CAGGCCCTCA TTGACCTGCA CAACCACGAC CTCGGGGAGA 1621 ACCACCACCT GCGGGTCTCC TTCTCCAAGT CCACCATCTA CCCAACTTTC TTGTACAAAG 1681 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1741 GAACTAGTCC GTAACTTGAA AGTTTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA 1801 CGAAAAACCT TCTTTATGTA CGACGAGACG CGTTAAGTCg acaatcaacc tctggattac 1861 aaaatttgtg aaagatt