Construct: ORF TRCN0000468225
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006491.1_s317c1
- Derived from:
- ccsbBroadEn_06845
- DNA Barcode:
- TTCACTTTGCTCGCTTAATGCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLAAT4 (5920)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468225
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5920 | PLAAT4 | phospholipase A and acyltra... | NM_004585.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 558
- ORF length:
- 492
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaaa 61 ttggcatggc ttcgccacac caagagccca aacctggaga cctgattgag attttccgcc 121 ttggctatga gcactgggcc ctgtatatag gagatGGCTA CGTGATCCAT CTGGCTCCTC 181 CAAGTGAGTA CCCCGGGGCT GGCTCCTCCA GTGTCTTCTC AGTCCTGAGC AACAGTGCAG 241 AGGTGAAACG GGAGCGCCTG GAAGATGTGG TGGGAGGCTG TTGCTATCGG GTCAACAACA 301 GCTTGGACCA TGAGTACCAA CCACGGCCCG TGGAGGTGAT CATCAGTTCT GCGAAGGAGA 361 TGGTTGGTCA GAAGATGAAG TACAGTATTG TGAGCAGGAA CTGTGAGCAC TTTGTCACCC 421 AGCTGAGATA TGGCAAGTCC CGCTGTAAAC AGGTGGAAAA GGCCAAGGTT GAAGTCGGTG 481 TGGCCACGGC GCTTGGAATC CTGGTTGTTG CTGGATGCTC TTTTGCGATT AGGAGATACC 541 AAAAAAAAGC GACAGCCTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATTCACTT TGCTCGCTTA 721 ATGCAGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt