Construct: ORF TRCN0000468226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005439.1_s317c1
- Derived from:
- ccsbBroadEn_13077
- DNA Barcode:
- ACAGACGCTGATGGCCGGTAATTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C11orf74 (119710)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468226
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NM_001276725.1 | 100% | 100% | |
| 2 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NM_001276726.1 | 100% | 100% | |
| 3 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NM_001276727.1 | 100% | 100% | |
| 4 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NM_001276722.1 | 66.5% | 66.5% | 136_357del |
| 5 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NM_001276723.1 | 66.5% | 66.5% | 136_357del |
| 6 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NM_001276724.1 | 66.5% | 66.5% | 136_357del |
| 7 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NM_138787.3 | 66.5% | 66.5% | 136_357del |
| 8 | human | 119710 | C11orf74 | chromosome 11 open reading ... | XM_006718138.4 | 66.5% | 66.5% | 136_357del |
| 9 | human | 119710 | C11orf74 | chromosome 11 open reading ... | XM_011519885.3 | 66.5% | 66.5% | 136_357del |
| 10 | human | 119710 | C11orf74 | chromosome 11 open reading ... | NR_077243.1 | 43.5% | 1_292del;427_581del;889_1013del | |
| 11 | human | 729930 | LOC729930 | chromosome 11 open reading ... | NR_077244.1 | 36.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgcccatatg tcaggattgg aaataatgga tgaagatcaa ttaatcaaag 121 acgtcttGGA TAAATTCCTT AATTGTCATG AGCAAACATA TGATGAAGAA TTTCTGAACA 181 CTTTTACTCA TCTTTCACAA GATTTGCTGC TGCTTCCAGG AGAAGTGGAG CAGGATGTAA 241 GCACCAGCAT TCCTTCCTGT ATCCCTTTTG TGGCCCAGCC TCCTACCTGT GAAGTGAAGC 301 CAAAGCCCAG TGTTAAAAGA ATGGACAAAC AGACGGAAGA GATACTTGGA GATGAAGTTC 361 AACTTTTTTC ACTTGATGAA GAATTTGATT ATGACAATGT GATGCTAACC TCCAAGTTTA 421 GTCCTGCAGA GATAGAGAAC ATCAAAGAGC TATGCAAGCA GCAGAAGAGA AAGGACACCA 481 GCCCAGACTT AGAGAAATCC TGTGACTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 541 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 601 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAACAGACGC 661 TGATGGCCGG TAATTGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 721 aagatt