Construct: ORF TRCN0000468267
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015214.1_s317c1
- Derived from:
- ccsbBroadEn_06082
- DNA Barcode:
- ATGTATAAGCAAGACATGTATCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGKG (1608)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468267
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080745.2 | 99.8% | 99.7% | (many diffs) |
| 2 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001346.3 | 94.9% | 94.8% | (many diffs) |
| 3 | human | 1608 | DGKG | diacylglycerol kinase gamma | NM_001080744.2 | 91.7% | 91.6% | (many diffs) |
| 4 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_001346673.1 | 87.1% | 89.2% | (many diffs) |
| 5 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | NM_138650.2 | 82.8% | 84.8% | (many diffs) |
| 6 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316851.1 | 82% | 84% | (many diffs) |
| 7 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316850.1 | 78.2% | 80.1% | (many diffs) |
| 8 | mouse | 110197 | Dgkg | diacylglycerol kinase, gamma | XM_017316852.1 | 68.6% | 69.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2325
- ORF length:
- 2256
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gggtgaagaa cggtgggtct ccctcactcc agaagaattt gaccaactcc 121 agaaatattc agaatattcc tccaagaaga taaaagatgc cttgactgaa tttaatgagg 181 gtgggagcct caaacaatat gacccacatg agccgattag ctatgatgtc ttcaagctgt 241 tcatgagggc gtacctagag gtggaccttc cccagccact gagcactcac ctcttcctgg 301 ccttcagcca gaagcccaga cacgagacct ctgaccaccc gacggaggga gccagcaaca 361 gtgaggccaa cagcgcagat actaatatac agaatgcaga taatgccacc aaagcagacg 421 aggcctgtgc ccctgatact gaatcaaata tggctgagaa gcaagcacca gctgaagacc 481 aagtggctgc gagccccctg gaaccccccg tccctcggtc ttcaagctcg gaatccccag 541 tggtgtacct gaaggatgtt gtgtgctacc tgtccctgct ggagacgggg aggcctcagg 601 ataagctgga gttcatgttt cgcctctatg attcagatga gaacggtctc ctggaccaag 661 cggagatgga ttgcattgtc aaccaaatgc tgcatattgc ccagtacctg gagtgggatc 721 ccacagagct gaggcctata ttgaaggaga tgctgcaagg gatggactac gaccgggacg 781 gctttgtgtc tctacaggaa tgggtccatg gagggatgac caccatccca ttgctggtcc 841 tcctggggat ggatgactct ggctccaagg gggatgggcg gcacgcctgg accatgaagc 901 acttcaagaa accaacctac tgcaacttct gccatatcat gctcatgggc gtccgcaagc 961 aaggcctgtg ctgcacttac tgtaaataca ctgtccacga acgctgtgtg tccaaaaaca 1021 ttcctggttg tgtcaaaacg tactcaaaag ccaaaaggag tggtgagttt caccgcaaat 1081 gtgaattatc aacgttgtgt gacggtgggg aactcagaga ccacatctta ctgcccacct 1141 ccatatgccc catcacccgg gacaggccag gtgagaagtc tgatggctgc gtgtccgcca 1201 agggcgaact tgtcatgcag tataagatca tccccacccc gggtacccac cccctgctgg 1261 tcttggtgaa ccccaagagt ggagggagac aaggagaaag aattcttcgg aaattccact 1321 atctgctcaa ccccaaacaa gttttcaacc tggacaatgg ggggcctact ccagggttga 1381 actttttccg tgatactcca gacttccgtg ttttggcctg tggtggagat gggacagttg 1441 gctggatttt ggattgcatt gataaggcca actttgcaaa gcatccacca gtggctgtcc 1501 tgcctcttgg aacaggaaat gaccttgccc gttgtctccg ctggggagga ggttatgaag 1561 ggggcagctt gacaaaaatc ctgaaagaca ttgagcagag ccccttggtg atgctggacc 1621 gctggcatct ggaagtcatc cccagagagg aagtggaaaa cggggaccag gtcccataca 1681 gcatcatgaa caactatttc tccattggtg tggacgcttc cattgcacac agattccatg 1741 tgatgagaga gaaacatcct gaaaaattca acagcaggat gaagaacaag ctgtggtact 1801 ttgaatttgg cacctcggag acttttgcag cgacctgcaa gaaactccac gaccacattg 1861 agttggagtg tgatggggtt ggggtggacc tgagcaacat cttcctggaa ggcattgcca 1921 ttctcaacat tcccagcatg tacggaggca ccaatctctg gggagaaaac aagaagaacc 1981 gggctgtgat ccgggaaagc aggaagggtg tCACTGACCC CAAAGAACTG AAATTCTGCG 2041 TTCAAGACCT CAGTGACCAG CTCCTTGAAG TGGTGGGGCT AGAAGGAGCC ATGGAGATGG 2101 GGCAGATCTA CACCGGCCTG AAGAGTGCAG GCAGGAGGCT GGCCCAGTGC GCCTCTGTCA 2161 CCATCAGGAC AAACAAGCTG CTGCCAATGC AAGTGGATGG AGAACCCTGG ATGCAGCCAT 2221 GTTGCACGAT TAAAATTACT CACAAGAACC AAGCGCCCAT GATGATGGGG CCTCCCCAGA 2281 AGAGCAGCTT CTTCTCGTTG AGAAGGAAGA GCCGTTCAAA AGACTTGCCA ACTTTCTTGT 2341 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2401 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2461 GAAAGGACGA ATGTATAAGC AAGACATGTA TCCGACGCGT TAAGTCgaca atcaacctct 2521 ggattacaaa atttgtgaaa gatt