Construct: ORF TRCN0000468276
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006373.1_s317c1
- Derived from:
- ccsbBroadEn_03234
- DNA Barcode:
- GATCTTACTCCAAACCGTTCTCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- HSD17B14 (51171)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468276
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51171 | HSD17B14 | hydroxysteroid 17-beta dehy... | NM_016246.3 | 100% | 100% | |
| 2 | human | 51171 | HSD17B14 | hydroxysteroid 17-beta dehy... | XM_005258969.4 | 78.5% | 68.2% | 541_542ins97;636_637ins77 |
| 3 | human | 51171 | HSD17B14 | hydroxysteroid 17-beta dehy... | XR_243938.3 | 57.5% | 1_275del;552_553ins92;994_1156del | |
| 4 | human | 51171 | HSD17B14 | hydroxysteroid 17-beta dehy... | XM_005258970.3 | 51.8% | 39.1% | 277_278ins92;420_421ins298 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 876
- ORF length:
- 810
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tacgggaacg cgctatgccg ggaaggtggt ggtcgtgacc gggggcgggc 121 gcggcatcgg agctgggatc gtgcgcgcct tcgtgaacag cggggcccga gtggttatct 181 gcgacaagga tgagtctggg ggccgggccc tggagcagga gctccctgga gctgtcttta 241 tcctctgtga tgtgactcag gaagatgatg tgaagaccct ggtttctgag accatccgcc 301 gatttggccg cctggattgt gttgtcaaca acgctggcca ccacccaccc ccacagaggc 361 ctgaggagac ctctgcccag ggattccgcc agctgctgga gctgaaccta ctggggacgt 421 acaccttgac caagctcgcc ctcccctacc tgcggaagag tcaagggaat gtcatcaaca 481 tctccagccT GGTGGGGGCA ATCGGCCAGG CCCAGGCAGT TCCCTATGTG GCCACCAAGG 541 GGGCAGTAAC AGCCATGACC AAAGCTTTGG CCCTGGATGA AAGTCCATAT GGTGTCCGAG 601 TCAACTGTAT CTCCCCAGGA AACATCTGGA CCCCGCTGTG GGAGGAGCTG GCAGCCTTAA 661 TGCCAGACCC TAGGGCCACA ATCCGAGAGG GCATGCTGGC CCAGCCACTG GGCCGCATGG 721 GCCAGCCCGC TGAGGTCGGG GCTGCGGCAG TGTTCCTGGC CTCCGAAGCC AACTTCTGCA 781 CGGGCATTGA ACTGCTCGTG ACGGGGGGTG CAGAGCTGGG GTACGGGTGC AAGGCCAGTC 841 GGAGCACCCC CGTGGACGCC CCCGATATCC CTTCCTGCCC AACTTTCTTG TACAAAGTGG 901 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 961 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1021 AGATCTTACT CCAAACCGTT CTCATACGCG TTAAGTCgac aatcaacctc tggattacaa 1081 aatttgtgaa agatt