Transcript: Human XM_005258970.3

PREDICTED: Homo sapiens hydroxysteroid 17-beta dehydrogenase 14 (HSD17B14), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
HSD17B14 (51171)
Length:
1058
CDS:
278..700

Additional Resources:

NCBI RefSeq record:
XM_005258970.3
NBCI Gene record:
HSD17B14 (51171)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005258970.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416336 CCGATATCCCTTCCTGATTTC pLKO_005 885 3UTR 100% 10.800 15.120 N HSD17B14 n/a
2 TRCN0000434289 CTTCTGCACGGGCATTGAACT pLKO_005 796 3UTR 100% 4.950 6.930 N HSD17B14 n/a
3 TRCN0000036678 TCAAGGGAATGTCATCAACAT pLKO.1 581 CDS 100% 4.950 6.930 N HSD17B14 n/a
4 TRCN0000036677 CCGAGTGGTTATCTGCGACAA pLKO.1 379 CDS 100% 4.050 5.670 N HSD17B14 n/a
5 TRCN0000036675 GCCCTGGATGAAAGTCCATAT pLKO.1 690 CDS 100% 10.800 7.560 N HSD17B14 n/a
6 TRCN0000429185 CCCAAACTCCAACCTGTATCA pLKO_005 947 3UTR 100% 4.950 3.465 N HSD17B14 n/a
7 TRCN0000036676 CCTGGATTGTGTTGTCAACAA pLKO.1 523 CDS 100% 4.950 3.465 N HSD17B14 n/a
8 TRCN0000036674 GCTGTCTTTATCCTCTGTGAT pLKO.1 443 CDS 100% 4.950 3.465 N HSD17B14 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005258970.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03234 pDONR223 100% 51.8% 39.1% None 277_278ins92;420_421ins298 n/a
2 ccsbBroad304_03234 pLX_304 0% 51.8% 39.1% V5 277_278ins92;420_421ins298 n/a
3 TRCN0000468276 GATCTTACTCCAAACCGTTCTCAT pLX_317 47.8% 51.8% 39.1% V5 277_278ins92;420_421ins298 n/a
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