Construct: ORF TRCN0000468278
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006935.1_s317c1
- Derived from:
- ccsbBroadEn_02506
- DNA Barcode:
- TATGGTTCGGTAGGTCACGCTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EBP (10682)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468278
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10682 | EBP | EBP cholestenol delta-isome... | NM_006579.3 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac taccaacgcg ggccccttgc acccatactg gcctcagcac ctaagactgg 121 acaactttgt acctaatgac cgccccacct ggcatatact ggctggcctc ttctctgtca 181 caggggtctt agtcgtgacc acatggctgt tgtcaggtcg tgctgcggtt gtcccattgg 241 ggacttggcg gcgactgtcc ctgtgctggt ttgcagtgtg tgggttcatt cacctggtga 301 tcgagggctg gttcgttctc tactacgaag acctgcttgg agacCAAGCC TTCTTATCTC 361 AACTCTGGAA AGAGTATGCC AAGGGAGACA GCCGATACAT CCTGGGTGAC AACTTCACAG 421 TGTGCATGGA AACCATCACA GCTTGCCTGT GGGGACCACT CAGCCTGTGG GTGGTGATCG 481 CCTTTCTCCG CCAGCATCCC CTCCGCTTCA TTCTACAGCT TGTGGTCTCT GTGGGCCAGA 541 TCTATGGGGA TGTGCTCTAC TTCCTGACAG AGCACCGCGA CGGATTCCAG CACGGAGAGC 601 TGGGCCACCC TCTCTACTTC TGGTTTTACT TTGTCTTCAT GAATGCCCTG TGGCTGGTGC 661 TGCCTGGAGT CCTTGTGCTT GATGCTGTGA AGCACCTCAC TCATGCCCAG AGCACGCTGG 721 ATGCCAAGGC CACAAAAGCC AAGAGCAAGA AGAACTGCCC AACTTTCTTG TACAAAGTGG 781 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 841 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 901 ATATGGTTCG GTAGGTCACG CTCCGACGCG TTAAGTCgac aatcaacctc tggattacaa 961 aatttgtgaa agatt