Transcript: Human NM_006579.3

Homo sapiens EBP cholestenol delta-isomerase (EBP), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
EBP (10682)
Length:
1125
CDS:
174..866

Additional Resources:

NCBI RefSeq record:
NM_006579.3
NBCI Gene record:
EBP (10682)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_006579.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000049394 CTCCGCTTCATTCTACAGCTT pLKO.1 609 CDS 100% 2.640 3.696 N EBP n/a
2 TRCN0000049397 CTGGACAACTTTGTACCTAAT pLKO.1 225 CDS 100% 10.800 7.560 N EBP n/a
3 TRCN0000333041 CTGGACAACTTTGTACCTAAT pLKO_005 225 CDS 100% 10.800 7.560 N EBP n/a
4 TRCN0000049395 CACCCTCTCTACTTCTGGTTT pLKO.1 714 CDS 100% 4.950 3.465 N EBP n/a
5 TRCN0000333043 CACCCTCTCTACTTCTGGTTT pLKO_005 714 CDS 100% 4.950 3.465 N EBP n/a
6 TRCN0000049393 GCACCTAAGACTGGACAACTT pLKO.1 215 CDS 100% 4.950 3.465 N EBP n/a
7 TRCN0000333040 GCACCTAAGACTGGACAACTT pLKO_005 215 CDS 100% 4.950 3.465 N EBP n/a
8 TRCN0000049396 CGTTCTCTACTACGAAGACCT pLKO.1 422 CDS 100% 2.640 1.848 N EBP n/a
9 TRCN0000333108 CGTTCTCTACTACGAAGACCT pLKO_005 422 CDS 100% 2.640 1.848 N EBP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_006579.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_02506 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_02506 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468278 TATGGTTCGGTAGGTCACGCTCCG pLX_317 59.4% 100% 100% V5 n/a
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