Construct: ORF TRCN0000468285
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017080.1_s317c1
- Derived from:
- ccsbBroadEn_12759
- DNA Barcode:
- TTTCAATACCGGGGCCGAACGTCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM120A (83862)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468285
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83862 | TMEM120A | transmembrane protein 120A | NM_001363462.2 | 92.2% | 91.6% | (many diffs) |
| 2 | human | 83862 | TMEM120A | transmembrane protein 120A | XM_024446953.1 | 78.4% | 55.8% | 257A>G;735_832del;1065_1230del |
| 3 | human | 83862 | TMEM120A | transmembrane protein 120A | NM_031925.3 | 76% | 56.4% | (many diffs) |
| 4 | human | 83862 | TMEM120A | transmembrane protein 120A | NM_001317803.2 | 71.1% | 48.6% | (many diffs) |
| 5 | human | 83862 | TMEM120A | transmembrane protein 120A | NR_156695.2 | 58.4% | (many diffs) | |
| 6 | mouse | 215210 | Tmem120a | transmembrane protein 120A | NM_172541.2 | 67% | 53% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1032
- ORF length:
- 966
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgca gcccccgccc ccgggcccgc tgggcgactg cctgcgggac tgggaggatc 121 tacagcagga cttccagaac atccaggaga cccatcggct ctaccgcctg aagctggagg 181 agctgaccaa acttcagaac aattgcacca gctccatcac gcggcagaag aagcggctcc 241 aggagctggc cctcgccctg aagaaatgca aaccctccct cccagcagag gccgaggggg 301 ccgcacagga gctggagaac cggatgaaag agcgccaagg cctcttcttt gacatggagg 361 cctatttgcc taagaagaat ggattgtacc tgagcctggt tctggggaac gtcaacgtca 421 cgctcctgag caagcaggct aagtttgcct acaaggacga gtatgagaag ttcaagctct 481 acctcaccat catcctcatc ctcatctcct tcacttgccg cttcctgctc aactccaggg 541 tgacagatgc tgccttcaac ttcctgctgg tctggtacta ctgcaccctg accatccggg 601 agagcatcct catcaacaac ggctcccggt gggcagggcg ggccctgagg gaggggagca 661 tggaatgggg tgccaggacc cTCCGGGAAG CAGGAACAGG CGCTGGGGGT GATGGTGGCT 721 CTCTGCTCCA GGATCAAAGG CTGGTGGGTG TTCCATCACT ACGTGTCCAC CTTCCTGTCG 781 GGAGTCATGC TGACGTGGTC CGACGGTCTC ATGTACCAGA AATTCCGGAA CCAATTCCTC 841 TCCTTTTCCA TGTACCAGAG CTTCGTGCAG TTTCTCCAGT ACTACTACCA GAGCGGCTGC 901 CTCTACCGCC TGCGGGCGCT GGGCGAGCGG CACACCATGG ACCTCACTGT GGAGGGCTTC 961 CAGTCCTGGA TGTGGCGGGG CCTCACCTTC CTGCTGCCTT TTCTTTTCTT TGGACACTTC 1021 TGGCAGCTTT TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1081 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1141 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT CAATACCGGG GCCGAACGTC 1201 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t