Construct: ORF TRCN0000468323
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010595.1_s317c1
- Derived from:
- ccsbBroadEn_10275
- DNA Barcode:
- TTTGACTGTAATAAATGTTTGGAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB2B (84932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468323
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84932 | RAB2B | RAB2B, member RAS oncogene ... | XM_017021712.1 | 94.9% | 92.7% | (many diffs) |
| 2 | human | 84932 | RAB2B | RAB2B, member RAS oncogene ... | NM_001163380.2 | 88.8% | 84.1% | 1_13del;48_91del |
| 3 | human | 84932 | RAB2B | RAB2B, member RAS oncogene ... | XM_017021710.1 | 82.8% | 83.6% | (many diffs) |
| 4 | human | 84932 | RAB2B | RAB2B, member RAS oncogene ... | NM_032846.4 | 69.9% | 66.2% | 1_151del;186_229del |
| 5 | human | 84932 | RAB2B | RAB2B, member RAS oncogene ... | NR_028074.2 | 15.7% | 1_238del;692_2870del | |
| 6 | mouse | 76338 | Rab2b | RAB2B, member RAS oncogene ... | NM_172601.3 | 63% | 61.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 519
- ORF length:
- 453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga aaacaaatca aactgcaaat ctgggatacg gggagcagct ggagcactgc 121 tggtgtacga CATTACAAGG CGTGAAACCT TCAACCACCT GACCTCATGG TTAGAGGATG 181 CCCGGCAGCA CTCTAGTTCC AACATGGTTA TCATGCTCAT TGGGAATAAG AGTGACCTAG 241 AGTCCCGCAG GGATGTGAAG AGAGAAGAAG GAGAGGCCTT TGCTAGGGAG CATGGACTTA 301 TATTCATGGA AACTTCAGCC AAAACAGCCT GCAATGTTGA AGAGGCCTTC ATTAACACAG 361 CCAAAGAAAT ATATAGGAAG ATCCAGCAGG GTTTATTTGA TGTCCACAAT GAGGCAAATG 421 GCATCAAGAT TGGGCCCCAA CAGTCAATTT CAACATCAGT GGGACCCAGT GCCTCCCAGC 481 GGAACTCTCG TGACATAGGG TCCAACTCTG GCTGCTGCTG CCCAACTTTC TTGTACAAAG 541 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 601 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 661 ACGATTTGAC TGTAATAAAT GTTTGGAGAC GCGTTAAGTC gacaatcaac ctctggatta 721 caaaatttgt gaaagatt