Construct: ORF TRCN0000468328
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011193.1_s317c1
- Derived from:
- ccsbBroadEn_07405
- DNA Barcode:
- ATGCATCTAATTTGCCTCGTGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LIPG (9388)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468328
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9388 | LIPG | lipase G, endothelial type | NM_006033.4 | 99.8% | 99.6% | 1144C>A;1427G>A |
| 2 | human | 9388 | LIPG | lipase G, endothelial type | XM_005258390.1 | 89.2% | 86.1% | (many diffs) |
| 3 | human | 9388 | LIPG | lipase G, endothelial type | NM_001308006.2 | 85% | 84.6% | 571_572ins222;922C>A;1205G>A |
| 4 | human | 9388 | LIPG | lipase G, endothelial type | XM_011526267.1 | 83.8% | 83.6% | 0_1ins240;904C>A;1187G>A |
| 5 | human | 9388 | LIPG | lipase G, endothelial type | XM_011526265.3 | 75.6% | 72.3% | (many diffs) |
| 6 | human | 9388 | LIPG | lipase G, endothelial type | XM_017026095.1 | 60.4% | 60.2% | 0_1ins591;553C>A;836G>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1566
- ORF length:
- 1500
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag caactccgtt cctctgctct gtttctggag cctctgctat tgctttgctg 121 cggggagccc cgtacctttt ggtccagagg gacggctgga agataagctc cacaaaccca 181 aagctacaca gactgaggtc aaaccatctg tgaggtttaa cctccgcacc tccaaggacc 241 cagagcatga aggatgctac ctctccgtcg gccacagcca gcccttagaa gactgcagtt 301 tcaacatgac agctaaaacc tttttcatca ttcacggatg gacgatgagc ggtatctttg 361 aaaactggct gcacaaactc gtgtcagccc tgcacacaag agagaaagac gccaatgtag 421 ttgtggttga ctggctcccc ctggcccacc agctttacac ggatgcggtc aataatacca 481 gggtggtggg acacagcatt gccaggatgc tcgactggct gcaggagaag gacgattttt 541 ctctcgggaa tgtccacttg atcggctaca gcctcggagc gcacgtggcc gggtatgcag 601 gcaacttcgt gaaaggaacg gtgggccgaa tcacaggttt ggatcctgcc gggcccatgt 661 ttgaaggggc cgacatccac aagaggctct ctccggacga tgcagatttt gtggatgtcc 721 tccacaccta cacgcgttcc ttcggcttga gcattggtat tcagatgcct gtgggccaca 781 ttgacatcta ccccaatggg ggtgacttcc agccaggctg tggactcaac gatgtcttgg 841 gatcaattgc atatggaaca atcacagagg tggtaaaatg tgagcatgag cgagccgtcc 901 acctctttgt tgactctctg gtgaatcagg acaagccgag ttttgccttc cagtgcactg 961 actccaatcg cttcaaaaag gggatctgtc tgagctgccg caagaaccgt tgtaatagca 1021 ttggctacaa tgccaagaaa atgaggaaca agaggaacag caaaatgtac ctaaaaaccc 1081 gggcaggcat gcctttcaga gtttaccatt atcagatgaa aatccatgtc ttcagttaca 1141 agaacatggg agaaattgag cccacctttt ACGTCACCCT TTATGGCACT AATGCAGATT 1201 CCCAGACTAT GCCACTGGAA ATAGTGGAGC GGATCGAGCA GAATGCCACC AACACCTTCC 1261 TGGTCTACAC CGAGGAGGAC TTGGGAGACC TCTTGAAGAT CCAGCTCACC TGGGAGGGGG 1321 CCTCTCAGTC TTGGTACAAC CTGTGGAAGG AGTTTCGCAG CTACCTGTCT CAACCCCGCA 1381 ACCCCGGACG GGAGCTGAAT ATCAGGCGCA TCCGGGTGAA GTCTGGGGAA ACCCAGCGGA 1441 AACTGACATT TTGTACAGAA GACCCTGAGA ACACCAGCAT ATCCCCAGGC CAGGAGCTCT 1501 GGTTTCGCAA GTGTCGGGAT GGCTGGAGGA TGAAAAACGA AACCAGTCCC ACTGTGGAGC 1561 TTCCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1621 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1681 CTTGGCTTTA TATATCTTGT GGAAAGGACG AATGCATCTA ATTTGCCTCG TGCACACGCG 1741 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt