Construct: ORF TRCN0000468336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004734.1_s317c1
- Derived from:
- ccsbBroadEn_01068
- DNA Barcode:
- GCAGATTTATCCTAGCCGCGTTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NDUFA9 (4704)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468336
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4704 | NDUFA9 | NADH:ubiquinone oxidoreduct... | NM_005002.5 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggctgccgca caatcccggg ttgtccgggt cctgtcaatg tcacgttctg 121 ccattactgc aatagccaca tctgtgtgtc acggcccacc ctgtcgccag cttcatcatg 181 ccctcatgcc tcatgggaaa ggtggacgtt cctcagtcag tgggattgtg gccactgtgt 241 ttggagcaac aggattcctg gggcgatatg ttgtcaacca ccttggacgc atggggtcac 301 aggtaatcat accctatcgg tgtgataaat atgacatcat gcaccttcgt cccatgggtg 361 acctgggcca gcttctgttt ctggaatggg acgcgagaga taaagattct atccgacgag 421 tagtacaaca cagcaatgtg gtcatcaatc ttattggacg agactgggaa accaaaaact 481 ttgattttga ggatgttttt gtgaagattc cccaagcaat tgctcaactg tccaaggaag 541 ctggagttga aaaattcatt catgtttcac atctgaatgc gaatattaaa agctcttcta 601 gatatttgag aaataaggct gttggagaga aagtagtgag agatgcattt ccggaagcca 661 ttatcgtaaa gccgtcggac atctttggaa gagaggatag attccttaat tcttttgcaa 721 gtatgcatcg gtttggtcct ataccccttg gttccttggg ctggaagaca gttaaacaac 781 cagtatatgt cgtagatgta tccaaaggaa ttgttaatgc agttaaggat cctgatgcca 841 atgggaaatc ctttgctttc gttggtccca gtcggtacct ccttttccac ctggtgaagt 901 acatctttgc tgtggctcac agattgttcc tcccattccc cttgccgctt tttgcctatc 961 gatgggtagc aagagtcttt gaaataagcc catttgagcc ctggataaca agggataaag 1021 tggagcggat gcacatcaca gacatgaaat tgcctcacct gcctggctta gaagaccttg 1081 gtattcaggc aacaccaCTG GAACTCAAGG CCATTGAGGT GCTGCGGCGT CATCGCACTT 1141 ACCGCTGGCT GTCTGCTGAA ATTGAGGATG TGAAGCCGGC CAAGACCGTC AACATTTACC 1201 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1261 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1321 ATATATCTTG TGGAAAGGAC GAGCAGATTT ATCCTAGCCG CGTTGAACGC GTTAAGTCga 1381 caatcaacct ctggattaca aaatttgtga aagatt