Transcript: Human NM_005002.5

Homo sapiens NADH:ubiquinone oxidoreductase subunit A9 (NDUFA9), mRNA.

Source:
NCBI, updated 2019-08-22
Taxon:
Homo sapiens (human)
Gene:
NDUFA9 (4704)
Length:
8356
CDS:
14..1147

Additional Resources:

NCBI RefSeq record:
NM_005002.5
NBCI Gene record:
NDUFA9 (4704)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_005002.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000027182 CGCGAGAGATAAAGATTCTAT pLKO.1 340 CDS 100% 5.625 7.875 N NDUFA9 n/a
2 TRCN0000338671 CGCGAGAGATAAAGATTCTAT pLKO_005 340 CDS 100% 5.625 7.875 N NDUFA9 n/a
3 TRCN0000027207 CGAGTAGTACAACACAGCAAT pLKO.1 365 CDS 100% 4.950 6.930 N NDUFA9 n/a
4 TRCN0000338672 CGAGTAGTACAACACAGCAAT pLKO_005 365 CDS 100% 4.950 6.930 N NDUFA9 n/a
5 TRCN0000027152 GCCCTGGATAACAAGGGATAA pLKO.1 946 CDS 100% 10.800 8.640 N NDUFA9 n/a
6 TRCN0000338741 GCCCTGGATAACAAGGGATAA pLKO_005 946 CDS 100% 10.800 8.640 N NDUFA9 n/a
7 TRCN0000027163 CCAGTATATGTCGTAGATGTA pLKO.1 728 CDS 100% 4.950 3.465 N NDUFA9 n/a
8 TRCN0000338673 CCAGTATATGTCGTAGATGTA pLKO_005 728 CDS 100% 4.950 3.465 N NDUFA9 n/a
9 TRCN0000027134 TCACAGATTGTTCCTCCCATT pLKO.1 865 CDS 100% 4.050 2.835 N NDUFA9 n/a
10 TRCN0000165470 GCACCGCCCTTAATCCATTTA pLKO.1 3589 3UTR 100% 13.200 6.600 Y LOC389286 n/a
11 TRCN0000165057 GAGTGGACACAGCACATGTTT pLKO.1 3615 3UTR 100% 5.625 2.813 Y LOC389286 n/a
12 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 7061 3UTR 100% 4.950 2.475 Y ERAP2 n/a
13 TRCN0000021409 GCTGCCTTCAAGCATCTGTTT pLKO.1 3553 3UTR 100% 4.950 2.475 Y NEK5 n/a
14 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 4482 3UTR 100% 13.200 6.600 Y LIAS n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_005002.5, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01068 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01068 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468336 GCAGATTTATCCTAGCCGCGTTGA pLX_317 9.5% 100% 100% V5 n/a
4 ccsbBroadEn_13911 pDONR223 100% 99.8% 99.2% None 1115C>T;1125delC n/a
5 ccsbBroad304_13911 pLX_304 0% 99.8% 99.2% V5 (not translated due to frame shift) 1115C>T;1125delC n/a
6 TRCN0000473018 GGCCCAACTCCAAGCACAAAAAAG pLX_317 43.1% 99.8% 99.2% V5 (not translated due to frame shift) 1115C>T;1125delC n/a
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