Construct: ORF TRCN0000468352

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000047.1_s317c1
Derived from:
ccsbBroadEn_09597
DNA Barcode:
GCGGACCACGTTGTAAAAGAGCCC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KRT72 (140807)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468352
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 140807 KRT72 keratin 72 NM_001146225.2 99.9% 99.8% 1098T>A
2 human 140807 KRT72 keratin 72 NM_080747.3 99.9% 99.8% 1098T>A
3 human 140807 KRT72 keratin 72 NM_001146226.2 91.7% 91.5% 961_962ins126;972T>A
4 human 140807 KRT72 keratin 72 XM_017018834.1 83.8% 81.1% (many diffs)
5 human 140807 KRT72 keratin 72 XM_017018836.1 81.4% 80.8% (many diffs)
6 human 140807 KRT72 keratin 72 XM_017018833.1 80.7% 74.3% (many diffs)
7 human 140807 KRT72 keratin 72 XM_017018835.1 79.6% 73.1% (many diffs)
8 human 140807 KRT72 keratin 72 XM_024448846.1 63.1% 63% 0_1ins564;534T>A
9 human 140807 KRT72 keratin 72 XM_017018837.1 52.9% 52.8% 0_1ins720;378T>A
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1602
ORF length:
1533
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagccgccaa ctgacccatt tcccccgcgg ggagcgcctg ggcttcagcg
121gttgctccgc ggtcctctct ggcgggatcg gcagcagctc cgcctcattc cgggcccggg
181tcaagggctc ggcctccttt ggcagcaaga gcctctcctg ccttgggggc agccgaagcc
241tggcgctcag cgctgctgca cggcggggcg gcggccgcct gggcggcttc gtgggcaccg
301ccttcggcag cgccgggctg gggcccaagt gtccctccgt gtgcccaccc gggggcatcc
361ctcaggtcac cgtcaacaag agcctcctgg ccccgctcaa cgtggagatg gaccccgaga
421tccagagggt gcgcgcccag gagcgggagc agatcaaggc gctaaacaac aagttcgcct
481ccttcatcga caaggtgcgg ttcctggagc agcagaatca ggtgctagag accaagtgga
541acctcctaca gcagctggac ttgaacaact gcaggaagaa cctggagccc atttatgagg
601gctacatcag caacctgcag aagcagctgg agatgctgtc tggggacggg gtgaggctgg
661attcggagct gaggaacatg caggatttgg tggaggacta caagaagagg tatgaggtgg
721agattaacag acgcacagct gctgagaatg agtttgtggt gctcaagaag gacgtggatg
781ctgcttacat gaataaggtt gagctccagg ccaaggtgga ctccttgaca gatgagatta
841aattcttcaa gtgcctttat gaaggggaga tcactcagat ccagtcccac atcagcgaca
901cgtccatcgt cctgtcaatg gacaacaacc gggatctgga cctggacagc atcattgccg
961aggtccgtgc ccagtacgag gagattgccc taaagagcaa ggccgaggct gagaccctgt
1021accagaccaa gatccaggag ctgcaggtca cagcaggcca gcatggggat gacctcaagc
1081tcaccaaggc tgaaatctct gagctcaacc gcctgatcca gaggatccgc tcagagatag
1141ggaatgtgaa gaagcagtgt gccgaactgg agacggccat cgccgacgct gaacagcggg
1201gggactgcgc cctgaaagat gcccgggcca agctggatga gctggagggc gccctgcacc
1261aggccaagga ggagctggca cggatgcTGC GTGAGTACCA GGAGCTCGTG AGCCTGAAGC
1321TGGCCCTGGA TATGGAGATC GCCACCTACC GCAAGCTGCT GGAGAGCGAG GAGTGCAGGA
1381TGTCTGGCGA ATATCCAAAT TCTGTGAGCA TCTCCGTCAT CAGCAGCACC AATGCTGGGG
1441CAGGAGGGGC TGGCTTCAGC ATGGGCTTTG GCGCCTCAAG CAGTTATAGC TACAAAACTG
1501CAGCTGCAGA CGTCAAGACC AAAGGCAGCT GTGGCAGTGA GCTCAAGGAT CCCCTTGCCA
1561AAACCTCGGG GAGCAGCTGT GCCACCAAAA AGGCCTCCAG ATTGCCAACT TTCTTGTACA
1621AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT
1681AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA
1741AGGACGAGCG GACCACGTTG TAAAAGAGCC CACGCGTTAA GTCgacaatc aacctctgga
1801ttacaaaatt tgtgaaagat t

Download FASTA (ORF) (Full)