Construct: ORF TRCN0000468352
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000047.1_s317c1
- Derived from:
- ccsbBroadEn_09597
- DNA Barcode:
- GCGGACCACGTTGTAAAAGAGCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT72 (140807)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468352
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 140807 | KRT72 | keratin 72 | NM_001146225.2 | 99.9% | 99.8% | 1098T>A |
| 2 | human | 140807 | KRT72 | keratin 72 | NM_080747.3 | 99.9% | 99.8% | 1098T>A |
| 3 | human | 140807 | KRT72 | keratin 72 | NM_001146226.2 | 91.7% | 91.5% | 961_962ins126;972T>A |
| 4 | human | 140807 | KRT72 | keratin 72 | XM_017018834.1 | 83.8% | 81.1% | (many diffs) |
| 5 | human | 140807 | KRT72 | keratin 72 | XM_017018836.1 | 81.4% | 80.8% | (many diffs) |
| 6 | human | 140807 | KRT72 | keratin 72 | XM_017018833.1 | 80.7% | 74.3% | (many diffs) |
| 7 | human | 140807 | KRT72 | keratin 72 | XM_017018835.1 | 79.6% | 73.1% | (many diffs) |
| 8 | human | 140807 | KRT72 | keratin 72 | XM_024448846.1 | 63.1% | 63% | 0_1ins564;534T>A |
| 9 | human | 140807 | KRT72 | keratin 72 | XM_017018837.1 | 52.9% | 52.8% | 0_1ins720;378T>A |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1602
- ORF length:
- 1533
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagccgccaa ctgacccatt tcccccgcgg ggagcgcctg ggcttcagcg 121 gttgctccgc ggtcctctct ggcgggatcg gcagcagctc cgcctcattc cgggcccggg 181 tcaagggctc ggcctccttt ggcagcaaga gcctctcctg ccttgggggc agccgaagcc 241 tggcgctcag cgctgctgca cggcggggcg gcggccgcct gggcggcttc gtgggcaccg 301 ccttcggcag cgccgggctg gggcccaagt gtccctccgt gtgcccaccc gggggcatcc 361 ctcaggtcac cgtcaacaag agcctcctgg ccccgctcaa cgtggagatg gaccccgaga 421 tccagagggt gcgcgcccag gagcgggagc agatcaaggc gctaaacaac aagttcgcct 481 ccttcatcga caaggtgcgg ttcctggagc agcagaatca ggtgctagag accaagtgga 541 acctcctaca gcagctggac ttgaacaact gcaggaagaa cctggagccc atttatgagg 601 gctacatcag caacctgcag aagcagctgg agatgctgtc tggggacggg gtgaggctgg 661 attcggagct gaggaacatg caggatttgg tggaggacta caagaagagg tatgaggtgg 721 agattaacag acgcacagct gctgagaatg agtttgtggt gctcaagaag gacgtggatg 781 ctgcttacat gaataaggtt gagctccagg ccaaggtgga ctccttgaca gatgagatta 841 aattcttcaa gtgcctttat gaaggggaga tcactcagat ccagtcccac atcagcgaca 901 cgtccatcgt cctgtcaatg gacaacaacc gggatctgga cctggacagc atcattgccg 961 aggtccgtgc ccagtacgag gagattgccc taaagagcaa ggccgaggct gagaccctgt 1021 accagaccaa gatccaggag ctgcaggtca cagcaggcca gcatggggat gacctcaagc 1081 tcaccaaggc tgaaatctct gagctcaacc gcctgatcca gaggatccgc tcagagatag 1141 ggaatgtgaa gaagcagtgt gccgaactgg agacggccat cgccgacgct gaacagcggg 1201 gggactgcgc cctgaaagat gcccgggcca agctggatga gctggagggc gccctgcacc 1261 aggccaagga ggagctggca cggatgcTGC GTGAGTACCA GGAGCTCGTG AGCCTGAAGC 1321 TGGCCCTGGA TATGGAGATC GCCACCTACC GCAAGCTGCT GGAGAGCGAG GAGTGCAGGA 1381 TGTCTGGCGA ATATCCAAAT TCTGTGAGCA TCTCCGTCAT CAGCAGCACC AATGCTGGGG 1441 CAGGAGGGGC TGGCTTCAGC ATGGGCTTTG GCGCCTCAAG CAGTTATAGC TACAAAACTG 1501 CAGCTGCAGA CGTCAAGACC AAAGGCAGCT GTGGCAGTGA GCTCAAGGAT CCCCTTGCCA 1561 AAACCTCGGG GAGCAGCTGT GCCACCAAAA AGGCCTCCAG ATTGCCAACT TTCTTGTACA 1621 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1681 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1741 AGGACGAGCG GACCACGTTG TAAAAGAGCC CACGCGTTAA GTCgacaatc aacctctgga 1801 ttacaaaatt tgtgaaagat t