Transcript: Human NM_001146225.2

Homo sapiens keratin 72 (KRT72), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-07-07
Taxon:
Homo sapiens (human)
Gene:
KRT72 (140807)
Length:
1813
CDS:
12..1547

Additional Resources:

NCBI RefSeq record:
NM_001146225.2
NBCI Gene record:
KRT72 (140807)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001146225.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416612 ATGTCTGGCGAATATCCAAAT pLKO_005 1323 CDS 100% 10.800 15.120 N KRT72 n/a
2 TRCN0000116779 CGCCTCAAGCAGTTATAGCTA pLKO.1 1415 CDS 100% 3.000 4.200 N KRT72 n/a
3 TRCN0000116778 CCTTGACAGATGAGATTAAAT pLKO.1 766 CDS 100% 15.000 10.500 N KRT72 n/a
4 TRCN0000418840 AGGTATGAGGTGGAGATTAAC pLKO_005 651 CDS 100% 13.200 9.240 N KRT72 n/a
5 TRCN0000116781 CTCCTTGACAGATGAGATTAA pLKO.1 764 CDS 100% 13.200 9.240 N KRT72 n/a
6 TRCN0000433245 GACGTGGATGCTGCTTACATG pLKO_005 714 CDS 100% 4.950 3.465 N KRT72 n/a
7 TRCN0000415254 AGGCGCTAAACAACAAGTTCG pLKO_005 400 CDS 100% 4.050 2.835 N KRT72 n/a
8 TRCN0000116780 GCTCAGAGATAGGGAATGTGA pLKO.1 1072 CDS 100% 3.000 2.100 N KRT72 n/a
9 TRCN0000428998 GATTTGGTGGAGGACTACAAG pLKO_005 627 CDS 100% 4.950 2.970 N KRT72 n/a
10 TRCN0000116954 CCTCCTTCATCGACAAGGTAT pLKO.1 421 CDS 100% 4.950 2.475 Y KRT8P11 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001146225.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_09597 pDONR223 99.8% 99.9% 99.8% None 1098T>A n/a
2 ccsbBroad304_09597 pLX_304 0% 99.9% 99.8% V5 1098T>A n/a
3 TRCN0000468352 GCGGACCACGTTGTAAAAGAGCCC pLX_317 20.8% 99.9% 99.8% V5 1098T>A n/a
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