Construct: ORF TRCN0000468355
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001179.1_s317c1
- Derived from:
- ccsbBroadEn_08576
- DNA Barcode:
- AGCTCAACAGCGTTGGACCACGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PARVA (55742)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468355
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55742 | PARVA | parvin alpha | NM_018222.5 | 99.8% | 99.4% | 32T>C;1110C>G |
| 2 | human | 55742 | PARVA | parvin alpha | XM_005253015.3 | 87.9% | 87.6% | 0_1ins133;2delT;978C>G |
| 3 | mouse | 57342 | Parva | parvin, alpha | NM_020606.5 | 90.1% | 97.8% | (many diffs) |
| 4 | mouse | 57342 | Parva | parvin, alpha | XM_006508050.1 | 81% | 86.8% | (many diffs) |
| 5 | mouse | 57342 | Parva | parvin, alpha | XM_006508051.1 | 77.5% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1182
- ORF length:
- 1116
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc cacctccccg cagaagtcgc cttctgcccc caagtctccc actcccaagt 121 cgcccccgtc ccgcaagaaa gatgattcct tcttggggaa actcggaggg accctggccc 181 ggaggaagaa agccaaggag gtgtccgagc tgcaggagga gggaatgaac gccatcaacc 241 tgcccctcag cccaattccc tttgagctgg accccgagga cacgatgctg gaggagaatg 301 aggtgcgaac aatggtggat ccaaactcac gcagtgaccc caagcttcaa gaactgatga 361 aggtattaat tgactggatt aatgatgtgt tggttggaga aagaatcatt gtgaaagacc 421 tagctgaaga tttgtatgat ggacaagtcc tgcagaagct tttcgagaaa ctggagagtg 481 agaagctaaa tgtggctgag gtcacccagt cagagattgc tcagaagcaa aaactgcaga 541 ctgtcctgga gaagatcaat gaaaccctga aacttcctcc caggagcatc aagtggaatg 601 tggattctgt tcatgccaag agcctggtgg ccatcttaca cctgctcgtt gctctgtctc 661 agtatttccg cgcaccaatt cgactcccag accatgtttc catccaagtg gttgtggtcc 721 agaaacgaga aggaatcctc cagtctcggc aaatccaaga ggaaataact ggtaacacag 781 aggctctttc CGGGAGGCAT GAACGTGATG CCTTTGACAC CTTGTTCGAC CATGCCCCAG 841 ACAAGCTGAA TGTGGTGAAA AAGACACTCA TCACTTTCGT GAACAAGCAC CTGAATAAAC 901 TGAACCTGGA GGTCACAGAA CTGGAAACCC AGTTTGCAGA TGGGGTGTAC CTGGTGCTGC 961 TCATGGGGCT CCTGGAGGGC TACTTTGTGC CCCTGCACAG CTTCTTCCTG ACCCCGGACA 1021 GCTTTGAACA GAAGGTCTTG AATGTCTCCT TTGCCTTTGA GCTCATGCAA GATGGAGGGT 1081 TGGAAAAGCC AAAACCGCGG CCAGAAGACA TAGTCAACTG TGACCTGAAA TCTACACTAC 1141 GAGTGTTGTA CAACCTCTTC ACCAAGTACC GTAAGGTGGA GTGCCCAACT TTCTTGTACA 1201 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1261 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1321 AGGACGAAGC TCAACAGCGT TGGACCACGA AACGCGTTAA GTCgacaatc aacctctgga 1381 ttacaaaatt tgtgaaagat t