Construct: ORF TRCN0000468377
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007451.1_s317c1
- Derived from:
- ccsbBroadEn_13230
- DNA Barcode:
- CAAACTAGACGCAGGGACGGTCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C15orf32 (145858)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468377
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 145858 | C15orf32 | chromosome 15 open reading ... | NR_161370.1 | 28.6% | 1_472del;968_1726del | |
| 2 | human | 145858 | C15orf32 | chromosome 15 open reading ... | NR_161371.1 | 28% | 1_472del;968_1764del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 564
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacaaaaga acaagtgtgg atgcttcaaa ggaagacttg cacccggctg 121 acccccagag tggggaaggg gtcccaccTA ATAGGAAGAA CACCAAGACT TCCCCAAGAG 181 GAGAAGGAAC GGCTCCCCCT TTCTCGGCGA GGCCATGTGT CTGGACCCTG TGTGAGATGC 241 TTTCTATACT AGCCCTTGTT GGGGTGTTGC ACCCATTTTA CAGATCAAAC AACCAAGTTT 301 ATCAGAAATT AAAGACTCAT TTAAGATGCC AAAGCTCACG AGTGGATGGT TTGATGCTGA 361 AGCCAACATT GTTAACACCA TCTCAGCTCA AAAGCCCTGA AGGCCACTTA ATTCTACCAA 421 CCTTCAACCA TCTTGTCATA AGACACATCC TCGACCCAAA GCAGATATTC TGTGTAGCTG 481 ATGTGTGTAC AGATTGCAAA TTCAATTGCG GAAGCATAGA GAGGCACCAG AAGAGGCATC 541 TTATGAGAGT CAGCCAGGAC TGGTTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 601 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 661 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAC AAACTAGACG 721 CAGGGACGGT CCGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 781 att