Construct: ORF TRCN0000468379
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008417.1_s317c1
- Derived from:
- ccsbBroadEn_04919
- DNA Barcode:
- CATTGACATGCTACCCCTCCCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PTRH1 (138428)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468379
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 138428 | PTRH1 | peptidyl-tRNA hydrolase 1 h... | NM_001002913.3 | 100% | 100% | |
| 2 | human | 138428 | PTRH1 | peptidyl-tRNA hydrolase 1 h... | NM_001345977.1 | 85.5% | 85.5% | 462_463ins93 |
| 3 | human | 138428 | PTRH1 | peptidyl-tRNA hydrolase 1 h... | NM_001345978.1 | 85% | 68.7% | 416_417ins46;546_547ins50 |
| 4 | human | 138428 | PTRH1 | peptidyl-tRNA hydrolase 1 h... | NM_001345979.1 | 76.6% | 50.4% | 315_316ins100;492_493ins50 |
| 5 | human | 138428 | PTRH1 | peptidyl-tRNA hydrolase 1 h... | NM_001345980.1 | 56% | 51.8% | 316_317ins146;360_361ins136 |
| 6 | human | 138428 | PTRH1 | peptidyl-tRNA hydrolase 1 h... | XR_002956760.1 | 43.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gccgggcggc tttttgggcg ccggacagcg gctgagtaga gccatgagcc 121 gatgtgtttt ggagcctcgc cccccgggga agcggtggat ggtggctggc ctggggaatc 181 ccggactgcc cggcacgcga cacagcgtgg gcatggcggt gctggggcag ctggcgcggc 241 ggctgggtgt ggcggagagt tGGACGCGCG ACCGGCACTG TGCCGCCGAC CTCGCCCTGG 301 CCCCGCTGGG GGATGCCCAA CTGGTCCTGC TCCGGCCACG GCGGCTTATG AACGCCAACG 361 GGCGCAGCGT GGCCCGGGCT GCGGAGCTGT TTGGGCTGAC TGCCGAGGAA GTCTACCTGG 421 TGCATGATGA GCTGGACAAG CCCCTGGGGA GACTGGCTCT GAAGCTGGGG GGCAGTGCCA 481 GGGGCCACAA TGGAGTCCGT TCCTGCATTA GCTGCCTCAA CTCCAATGCA ATGCCAAGGC 541 TGCGGGTGGG TATCGGGCGC CCGGCGCACC CTGAGGCGGT TCAGGCCCAT GTGCTGGGCT 601 GCTTCTCCCC TGCTGAGCAG GAGCTGCTGC CTCTGTTGCT GGATCGAGCC ACCGACCTGA 661 TCTTGGACCA CATCCGTGAG CGAAGCCAGG GGCCCTCACT GGGGCCGTGC CCAACTTTCT 721 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 781 CGTAGTAATG AACTAGCCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 841 GTGGAAAGGA CGACATTGAC ATGCTACCCC TCCCAGCACG CGTTAAGTCg acaatcaacc 901 tctggattac aaaatttgtg aaagatt