Construct: ORF TRCN0000468392
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018247.1_s317c1
- Derived from:
- ccsbBroadEn_00129
- DNA Barcode:
- AAACCCAGGTCCTGTAAATTTACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ATP5F1D (513)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468392
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 513 | ATP5F1D | ATP synthase F1 subunit delta | NM_001001975.2 | 100% | 100% | |
| 2 | human | 513 | ATP5F1D | ATP synthase F1 subunit delta | NM_001687.5 | 100% | 100% | |
| 3 | mouse | 66043 | Atp5d | ATP synthase, H+ transporti... | NM_025313.2 | 79.9% | 86.3% | (many diffs) |
| 4 | mouse | 66043 | Atp5d | ATP synthase, H+ transporti... | XM_017314052.1 | 79.9% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 570
- ORF length:
- 504
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct gcccgccgcg ctgctccgcc gcccgggact tggccgcctc gtccgccacg 121 cccgtgccta tgccgaggcc gccgccgccc cggctgccgc ctctggcccc aaccagatgt 181 ccttcacctt cgcctctccc acgcaggtgt tcttcaacgg tgccaacgtc cggcaggtgg 241 acgtgcccac gctgaccgga gccTTCGGCA TCCTGGCGGC CCACGTGCCC ACGCTGCAGG 301 TCCTGCGGCC GGGGCTGGTC GTGGTGCATG CAGAGGACGG CACCACCTCC AAATACTTTG 361 TGAGCAGCGG TTCCATCGCA GTGAACGCCG ACTCTTCGGT GCAGTTGTTG GCCGAAGAGG 421 CCGTGACGCT GGACATGTTG GACCTGGGGG CAGCCAAGGC AAACTTGGAG AAGGCCCAGG 481 CGGAGCTGGT GGGGACAGCT GACGAGGCCA CGCGGGCAGA GATCCAGATC CGAATCGAGG 541 CCAACGAGGC CCTGGTGAAG GCCCTGGAGT ACCCAACTTT CTTGTACAAA GTGGTTGATA 601 TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA TGAACTAGTC 661 CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG GACGAAAACC 721 CAGGTCCTGT AAATTTACCA CGCGTTAAGT Cgacaatcaa cctctggatt acaaaatttg 781 tgaaagatt