Construct: ORF TRCN0000468396

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010651.1_s317c1
Derived from:
ccsbBroadEn_01225
DNA Barcode:
CGGCATTCCGTCAATTGCCGAACT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PMCH (5367)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468396
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5367 PMCH pro-melanin concentrating h... NM_002674.4 100% 100%
2 human 5367 PMCH pro-melanin concentrating h... XM_017019483.2 91.1% 90.3% 449delG;451_452insCTC;453_454ins40
3 human 5370 PMCHL2 pro-melanin concentrating h... NR_003922.1 12% (many diffs)
4 mouse 110312 Pmch pro-melanin-concentrating h... NM_029971.2 88.6% 86.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
561
ORF length:
495
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc aaaaatgaat ctctcttcct atatattaat actaactttt tctttgtttt
121ctcaaggtat tttactttca gcatccaagt ccataagaaa tttagatgat gacatggtat
181ttaatacatt caggttgggg aaaggctttc agaaggaaga cactgcagaa aaatcagtta
241ttgctccttc cctggaaCAA TATAAAAATG ATGAGAGCAG TTTCATGAAC GAAGAGGAAA
301ATAAAGTTTC AAAGAACACA GGCTCCAAAC ATAATTTCTT AAATCATGGT CTGCCACTGA
361ATCTGGCTAT AAAACCTTAT CTTGCACTAA AAGGATCTGT AGCTTTCCCA GCTGAGAATG
421GAGTTCAGAA TACTGAATCA ACACAAGAAA AGAGAGAAAT TGGGGATGAA GAAAACTCAG
481CTAAATTTCC TATAGGAAGG AGAGATTTTG ACATGCTCAG ATGTATGCTG GGAAGAGTCT
541ACCGACCTTG TTGGCAAGTC TGCACAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC
601CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG
661AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGGC ATTCCGTCAA
721TTGCCGAACT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt

Download FASTA (ORF) (Full)