Construct: ORF TRCN0000468396
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010651.1_s317c1
- Derived from:
- ccsbBroadEn_01225
- DNA Barcode:
- CGGCATTCCGTCAATTGCCGAACT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PMCH (5367)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468396
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5367 | PMCH | pro-melanin concentrating h... | NM_002674.4 | 100% | 100% | |
| 2 | human | 5367 | PMCH | pro-melanin concentrating h... | XM_017019483.2 | 91.1% | 90.3% | 449delG;451_452insCTC;453_454ins40 |
| 3 | human | 5370 | PMCHL2 | pro-melanin concentrating h... | NR_003922.1 | 12% | (many diffs) | |
| 4 | mouse | 110312 | Pmch | pro-melanin-concentrating h... | NM_029971.2 | 88.6% | 86.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 561
- ORF length:
- 495
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc aaaaatgaat ctctcttcct atatattaat actaactttt tctttgtttt 121 ctcaaggtat tttactttca gcatccaagt ccataagaaa tttagatgat gacatggtat 181 ttaatacatt caggttgggg aaaggctttc agaaggaaga cactgcagaa aaatcagtta 241 ttgctccttc cctggaaCAA TATAAAAATG ATGAGAGCAG TTTCATGAAC GAAGAGGAAA 301 ATAAAGTTTC AAAGAACACA GGCTCCAAAC ATAATTTCTT AAATCATGGT CTGCCACTGA 361 ATCTGGCTAT AAAACCTTAT CTTGCACTAA AAGGATCTGT AGCTTTCCCA GCTGAGAATG 421 GAGTTCAGAA TACTGAATCA ACACAAGAAA AGAGAGAAAT TGGGGATGAA GAAAACTCAG 481 CTAAATTTCC TATAGGAAGG AGAGATTTTG ACATGCTCAG ATGTATGCTG GGAAGAGTCT 541 ACCGACCTTG TTGGCAAGTC TGCACAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 601 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 661 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACGGC ATTCCGTCAA 721 TTGCCGAACT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt