Transcript: Human NR_003922.1

Homo sapiens pro-melanin concentrating hormone like 2 (pseudogene) (PMCHL2), non-coding RNA.

Source:
NCBI, updated 2018-05-24
Taxon:
Homo sapiens (human)
Gene:
PMCHL2 (5370)
Length:
1842
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_003922.1
NBCI Gene record:
PMCHL2 (5370)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_003922.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000148063 GATACCGAATCAACACTAGAA pLKO.1 148 3UTR 100% 4.950 2.970 N PMCHL2 n/a
2 TRCN0000149528 GCTATCGTATTTGGAGTGGAA pLKO.1 702 3UTR 100% 2.640 1.584 N PMCHL2 n/a
3 TRCN0000151851 CTGTCACTGAATCTGGTTATA pLKO.1 70 3UTR 100% 13.200 6.600 Y PMCHL1 n/a
4 TRCN0000148791 CTTGCACTAGAAGGATCTGTA pLKO.1 100 3UTR 100% 4.950 2.475 Y PMCHL2 n/a
5 TRCN0000149356 GCCTGTGTTCTGTAAAGAGAA pLKO.1 664 3UTR 100% 4.950 2.475 Y PMCHL2 n/a
6 TRCN0000153159 GCCTGTGTTCTGTAAAGAGAA pLKO.1 664 3UTR 100% 4.950 2.475 Y PMCHL1 n/a
7 TRCN0000149442 GTTCAGGATACCGAATCAACA pLKO.1 142 3UTR 100% 4.950 2.475 Y PMCHL2 n/a
8 TRCN0000153002 GTTCAGGATACCGAATCAACA pLKO.1 142 3UTR 100% 4.950 2.475 Y PMCHL1 n/a
9 TRCN0000149822 CACTAGAAGGATCTGTAGCTT pLKO.1 104 3UTR 100% 3.000 1.500 Y PMCHL2 n/a
10 TRCN0000152483 CACTAGAAGGATCTGTAGCTT pLKO.1 104 3UTR 100% 3.000 1.500 Y PMCHL1 n/a
11 TRCN0000152255 CTTAAATCATGGTCTGTCACT pLKO.1 57 3UTR 100% 2.640 1.320 Y PMCHL1 n/a
12 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1000 3UTR 100% 5.625 2.813 Y KLHL30 n/a
13 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1000 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_003922.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01225 pDONR223 100% 12% None (many diffs) n/a
2 ccsbBroad304_01225 pLX_304 0% 12% V5 (many diffs) n/a
3 TRCN0000468396 CGGCATTCCGTCAATTGCCGAACT pLX_317 60.9% 12% V5 (many diffs) n/a
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