Construct: ORF TRCN0000468403
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017550.1_s317c1
- Derived from:
- ccsbBroadEn_05327
- DNA Barcode:
- ACTGTGGCGTAATCGCCCACATCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAS2R31 (259290)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468403
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259290 | TAS2R31 | taste 2 receptor member 31 | NM_176885.2 | 100% | 100% | |
| 2 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | NM_176884.2 | 94.7% | 89.3% | (many diffs) |
| 3 | human | 259292 | TAS2R46 | taste 2 receptor member 46 | NM_176887.2 | 92.4% | 85.1% | (many diffs) |
| 4 | human | 259291 | TAS2R45 | taste 2 receptor member 45 | NM_176886.2 | 89.3% | 81.5% | (many diffs) |
| 5 | human | 259289 | TAS2R43 | taste 2 receptor member 43 | XM_003960991.5 | 89.3% | 81.5% | (many diffs) |
| 6 | human | 259293 | TAS2R30 | taste 2 receptor member 30 | NM_001097643.1 | 88.9% | 77.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 996
- ORF length:
- 927
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gacaactttt atacccatca ttttttccag tgtggtagtg gttctatttg 121 ttattggaaa ttttgctaat ggcttcatag cattggtaaa ttccattgag cgggtcaaga 181 gacaaaagat ctcttttgct gaccagattc tcactgctct ggcggtctcc agagttggtt 241 tgctctgggt attattatta aattggtatt caactgtgtt taatccagct ttttatagtg 301 tagaagtaag aactactgct tataatgtct gggcagtaac cggccatttc agcaactggc 361 ttgctactag cctcagcata ttttatttgc tcaagattgc caatttctcc aaccttattt 421 ttcttcactt aaagaggaga gttaagagtg tcattctggt gatgctgttg gggcctttac 481 tatttttggc ttgtcaactt tttgtgataa acatgaaaga gattgtacgg acaaaagaat 541 atgaaggaaa cttgacttgg aagatcaaat tgaggagtgc agtgtacctt tcagatgcga 601 ctgtaaccac gctaggaaac ttagtgccct tcactctgac cctgctatgt tttttgctgt 661 taatctgttc tctgtgtaaa CATCTCAAGA AGATGCAGCT CCATGGTAAA GGATCTCAAG 721 ATCCCAGCAC CAAGGTCCAC ATAAAAGCTT TGCAAACTGT GATCTTTTTC CTCTTGTTAT 781 GTGCCGTTTA CTTTCTGTCC ATAATGATAT CAGTTTGGAG TTTTGGGAGT CTGGAAAACA 841 AACCTGTCTT CATGTTCTGC AAAGCTATTA GATTCAGCTA TCCTTCAATC CACCCATTCA 901 TCCTGATTTG GGGAAACAAG AAGCTAAAGC AGACTTTTCT TTCAGTTTTG CGGCAAGTGA 961 GGTACTGGGT GAAAGGAGAG AAGCCTTCAT CTCCATTGCC AACTTTCTTG TACAAAGTGG 1021 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1081 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1141 AACTGTGGCG TAATCGCCCA CATCGACGCG TTAAGTCgac aatcaacctc tggattacaa 1201 aatttgtgaa agatt