Construct: ORF TRCN0000468417
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010955.1_s317c1
- Derived from:
- ccsbBroadEn_02049
- DNA Barcode:
- CTTTGCCTCGTGATCTTTACCCCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RTL8C (8933)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468417
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8933 | RTL8C | retrotransposon Gag like 8C | NM_001078171.2 | 100% | 100% | |
| 2 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001078172.1 | 95.2% | 93.8% | (many diffs) |
| 3 | human | 441518 | RTL8B | retrotransposon Gag like 8B | NM_001078173.2 | 94.3% | 92.9% | (many diffs) |
| 4 | human | 26071 | RTL8A | retrotransposon Gag like 8A | NM_001134321.2 | 59.2% | 50% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 429
- ORF length:
- 339
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGGACGGTCG GGTGCAGCTG ATAAAGGCCC 121 TCCTGGCCTT GCCGATCCGG CCTGCGACGC GTCGCTGGAG GAACCCGATT CCCTTTCCCG 181 AGACGTTTGA CGGCGATACC GACCGACTCC CGGAGTTCAT CGTGCAGACG GGCTCCTACA 241 TGTTCGTGGA CGAGAACACG TTCTCCAGCG ACGCCCTGAA GGTGACGTTC CTCATCACCC 301 GCCTCACAGG GCCCGCCCTG CAGTGGGTGA TCCCCTACAT CAAGAAGGAG AGCCCCCTCC 361 TCAATGATTA CCGGGGCTTT CTGGCCGAGA TGAAGCGAGT CTTTGGATGG GAGGAGGACG 421 AGGACTTCTA CCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT ATCCCTAACC 481 CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA AGTATTTCGA 541 TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGACTTTGC CTCGTGATCT TTACCCCTAC 601 GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt