Construct: ORF TRCN0000468448
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014637.1_s317c1
- Derived from:
- ccsbBroadEn_00253
- DNA Barcode:
- CCCACCGCTCAATTGTTCGTAGCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CD27 (939)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468448
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 939 | CD27 | CD27 molecule | NM_001242.4 | 100% | 100% | |
| 2 | human | 939 | CD27 | CD27 molecule | XM_011521042.3 | 73.1% | 63.7% | (many diffs) |
| 3 | human | 939 | CD27 | CD27 molecule | XM_017020234.1 | 72.5% | 63.1% | 659_770del;810A>G;893_1074del |
| 4 | human | 939 | CD27 | CD27 molecule | XM_017020232.1 | 64.1% | 56.2% | 659_911del;951A>G;1034_1215del |
| 5 | human | 939 | CD27 | CD27 molecule | XM_017020233.2 | 47.5% | 34.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 846
- ORF length:
- 780
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc acggccacat ccctggtggc tgtgcgttct ggggaccctg gtggggctct 121 cagctactcc agcccccaag agctgcccag agaggcacta ctgggctcag ggaaagctgt 181 gctgccagat gtgtgagcca ggaacattcc tcgtgaagga ctgtgaccag catagaaagg 241 ctgctcagtg tgatccttgc ataccggggg tctccttctc tcctgaccac cacacccggc 301 cccactgtga gagctgtcgg cactgtaact ctggtcttct cgttcgcaac tgcaccatca 361 ctgccaatgc tgagtgtgcc tgtcgcaatg gctggcagtg cagGGACAAG GAGTGCACCG 421 AGTGTGATCC TCTTCCAAAC CCTTCGCTGA CCGCTCGGTC GTCTCAGGCC CTGAGCCCAC 481 ACCCTCAGCC CACCCACTTA CCTTATGTCA GTGAGATGCT GGAGGCCAGG ACAGCTGGGC 541 ACATGCAGAC TCTGGCTGAC TTCAGGCAGC TGCCTGCCCG GACTCTCTCT ACCCACTGGC 601 CACCCCAAAG ATCCCTGTGC AGCTCCGATT TTATTCGCAT CCTTGTGATC TTCTCTGGAA 661 TGTTCCTTGT TTTCACCCTG GCCGGGGCCC TGTTCCTCCA TCAACGAAGG AAATATAGAT 721 CAAACAAAGG AGAAAGTCCT GTGGAGCCTG CAGAGCCTTG TCGTTACAGC TGCCCCAGGG 781 AGGAGGAGGG CAGCACCATC CCCATCCAGG AGGATTACCG AAAACCGGAG CCTGCCTGCT 841 CCCCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 901 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGCCCGTA ACTTGAAAGT ATTTCGATTT 961 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACCCACCGCT CAATTGTTCG TAGCCACGCG 1021 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt