Construct: ORF TRCN0000468450
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003415.1_s317c1
- Derived from:
- ccsbBroadEn_11981
- DNA Barcode:
- AGGCGAGCTCAATTTCCCCGCCAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MBTPS2 (51360)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468450
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51360 | MBTPS2 | membrane bound transcriptio... | NM_015884.4 | 63.1% | 61.8% | (many diffs) |
| 2 | mouse | 270669 | Mbtps2 | membrane-bound transcriptio... | NM_172307.3 | 58.5% | 59.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat tccggtgtcg ctggtggtgg tggtggtggg tggctggact gtcgtctacc 121 tgaccgactt ggtgctggag tcatctgtct attttaaaca ttcttatgaa gactggctgg 181 aaaacaacgg actgagcatc tcccctttcc acataagatg gcaaactgct gttttcaatc 241 gtgcctttta cagttgggga cggcggaaag caaggatgct ttaccagtgg ttcaattttg 301 gaatggtgtt tggcgtaatt gccatgttta gctcattttt tctccttgga aaaacgctga 361 tgcagacttt ggcacaaatg atggctgact ctccctcttc ttattcttcc tcctcttctt 421 cctcttcctc ctcttcttcc tcttcctctt cttcatcttc ttcctcttcc tcgcttcaca 481 atgaacaggt gttacaagtt gtggttcctg gtataaattt acccgtcaat caactgacct 541 atttcttcac ggcagttctc attagtggtg ttgtacatga aattggacat gggatagcag 601 ctattaggga acaagttcga tttaatggct ttgggatttt tctcttcatt atttatcctg 661 gagcatttgt tgatctgttc accactcatt tgcaacttat atcgccagtc cagcagctaa 721 ggatattttg tgcaggtatc tggcataatt ttgtccttgc actcttgggt attttagctc 781 ttgttctcct cccagtaatt ctcttgccat tttactacac tGGAGTTGGG GTGCTCATCA 841 CTGAAGTTGC TGAGGACTCT CCTGCCATTG GACCCAGAGG CCTTTTTGTG GGAGACCTTG 901 TCACCCATCT ACAGGATTGT CCTGTTACTA ATGTGCAAGA TTGGAATGAA TGTTTAGATA 961 CCATCGCCTA TGAGCCCCAA ATTGGTTACT GTATAAGTGC ATCAACTTTA CAGCAGTTAA 1021 GTTTCCCAGT TAGAGGTGTG TATATTTCCT CAATATACCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AAGGCGAGCT CAATTTCCCC GCCATACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt